Gammaretrovirus-mediated correction of SCID-X1 is associated with skewed vector integration site distribution in vivo

• Published in: The Journal of clinical investigation 2007-08, Vol.117 (8), p.2241-2249
• Main Authors: Schwarzwaelder, Kerstin, Howe, Steven J, Schmidt, Manfred, Brugman, Martijn H, Deichmann, Annette, Glimm, Hanno, Schmidt, Sonja, Prinz, Claudia, Wissler, Manuela, King, Douglas J S, Zhang, Fang, Parsley, Kathryn L, Gilmour, Kimberly C, Sinclair, Joanna, Bayford, Jinhua, Peraj, Rachel, Pike-Overzet, Karin, Staal, Frank J T, de Ridder, Dick, Kinnon, Christine, Abel, Ulrich, Wagemaker, Gerard, Gaspar, H Bobby, Thrasher, Adrian J, von Kalle, Christof
• Format: Article
• Language: English
• Subjects: Adult; Biomedical research; Care and treatment; CD3 Complex; Cell Proliferation; Cell Survival - genetics; Cell Survival - immunology; Child; Child, Preschool; Female; Follow-Up Studies; Gammaretrovirus; Genetic aspects; Genetic Vectors; Graft Survival - genetics; Graft Survival - immunology; Hematopoietic Stem Cell Transplantation; Hematopoietic Stem Cells - immunology; Humans; Infant; Lymphocytes; Male; Severe combined immunodeficiency; T-Lymphocytes - immunology; Transduction, Genetic; Transplantation, Autologous; Virus Integration; X-Linked Combined Immunodeficiency Diseases - genetics; X-Linked Combined Immunodeficiency Diseases - immunology; X-Linked Combined Immunodeficiency Diseases - therapy
• ISSN: 0021-9738; 1558-8238
• DOI: 10.1172/JCI31661

We treated 10 children with X-linked SCID (SCID-X1) using gammaretrovirus-mediated gene transfer. Those with sufficient follow-up were found to have recovered substantial immunity in the absence of any serious adverse events up to 5 years after treatment. To determine the influence of vector integration on lymphoid reconstitution, we compared retroviral integration sites (RISs) from peripheral blood CD3(+) T lymphocytes of 5 patients taken between 9 and 30 months after transplantation with transduced CD34(+) progenitor cells derived from 1 further patient and 1 healthy donor. Integration occurred preferentially in gene regions on either side of transcription start sites, was clustered, and correlated with the expression level in CD34(+) progenitors during transduction. In contrast to those in CD34(+) cells, RISs recovered from engrafted CD3(+) T cells were significantly overrepresented within or near genes encoding proteins with kinase or transferase activity or involved in phosphorus metabolism. Although gross patterns of gene expression were unchanged in transduced cells, the divergence of RIS target frequency between transduced progenitor cells and post-thymic T lymphocytes indicates that vector integration influences cell survival, engraftment, or proliferation.