Effects of age-dependent membrane transport changes on the homeostasis of senescent human red blood cells

• Published in: Blood 2007-08, Vol.110 (4), p.1334-1342
• Main Authors: Lew, Virgilio L., Daw, Nuala, Etzion, Zipora, Tiffert, Teresa, Muoma, Adaeze, Vanagas, Laura, Bookchin, Robert M.
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biological Transport; Blood coagulation. Blood cells; Cell Membrane - metabolism; Cell Membrane Permeability; Erythrocyte Aging; Erythrocyte Membrane - metabolism; Erythrocytes - cytology; Erythrocytes - drug effects; Erythrocytes - metabolism; Fundamental and applied biological sciences. Psychology; General aspects, investigation methods, hemostasis, fibrinolysis; Glycated Hemoglobin A - metabolism; Hematologic and hematopoietic diseases; Homeostasis; Humans; Medical sciences; Molecular and cellular biology; Plasma Membrane Calcium-Transporting ATPases - metabolism; Potassium - metabolism; Red Cells; Sodium - metabolism
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood-2006-11-057232

Little is known about age-related changes in red blood cell (RBC) membrane transport and homeostasis. We investigated first whether the known large variation in plasma membrane Ca2+ (PMCA) pump activity was correlated with RBC age. Glycated hemoglobin, Hb A1c, was used as a reliable age marker for normal RBCs. We found an inverse correlation between PMCA strength and Hb A1c content, indicating that PMCA activity declines monotonically with RBC age. The previously described subpopulation of high-Na+, low-density RBCs had the highest Hb A1c levels, suggesting it represents a late homeostatic condition of senescent RBCs. Thus, the normal densification process of RBCs with age must undergo late reversal, requiring a membrane permeability increase with net NaCl gain exceeding KCl loss. Activation of a nonselective cation channel, Pcat, was considered the key link in this density reversal. Investigation of Pcat properties showed that its most powerful activator was increased intracellular Ca2+. Pcat was comparably selective to Na+, K+, choline, and N-methyl-D-glucamine, indicating a fairly large, poorly selective cation permeability pathway. Based on these observations, a working hypothesis is proposed to explain the mechanism of progressive RBC densification with age and of the late reversal to a low-density condition with altered ionic gradients.