Cytokines differentially regulate ICAM‐1 and VCAM‐1 expression on human gingival fibroblasts

• Published in: Clinical and experimental immunology 2006-06, Vol.144 (3), p.494-502
• Main Authors: Hosokawa, Y., Hosokawa, I., Ozaki, K., Nakae, H., Matsuo, T.
• Format: Article
• Language: English
• Subjects: Basic Immunology; Biological and medical sciences; Cells, Cultured; Cytokines - pharmacology; Cytokines - physiology; Drug Synergism; Enzyme Inhibitors - pharmacology; Fibroblasts - metabolism; Flow Cytometry - methods; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Gene Expression Regulation - drug effects; Gingiva - cytology; Gingiva - metabolism; gingival fibroblasts; Humans; ICAM‐1; Imidazoles - pharmacology; Immunopathology; Intercellular Adhesion Molecule-1 - biosynthesis; Intercellular Adhesion Molecule-1 - genetics; Leupeptins - pharmacology; Medical sciences; Mitogen-Activated Protein Kinase Kinases - antagonists & inhibitors; NF-kappa B - antagonists & inhibitors; NF-kappa B - physiology; Pyridines - pharmacology; Recombinant Proteins - pharmacology; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA, Messenger - genetics; Vascular Cell Adhesion Molecule-1 - biosynthesis; Vascular Cell Adhesion Molecule-1 - genetics; VCAM‐1
• ISSN: 0009-9104; 1365-2249
• DOI: 10.1111/j.1365-2249.2006.03064.x

The expression of intercellular adhesion molecule‐1 (ICAM‐1) and vascular adhesion molecule‐1 (VCAM‐1) on human gingival fibroblasts (HGF) may be important for migration and retention of inflammatory cells in periodontally diseased tissue. This study aimed to assess which cytokines regulate ICAM‐1 and VCAM‐1 expression on HGF. Tumour necrosis factor (TNF)‐α and interferon (IFN)‐γ enhanced both ICAM‐1 and VCAM‐1 expression on HGF. Interleukin (IL)‐1β mainly up‐regulated ICAM‐1 expression. On the other hand, IL‐4 and IL‐13 enhanced only VCAM‐1 expression on HGF. IL‐10 did not modulate both ICAM‐1 and VCAM‐1 expression. Transforming growth factor (TGF)‐β1 enhanced ICAM‐1 expression. However, TGF‐β1 inhibited the VCAM‐1 expression induced by TNF‐α or IL‐4. Both ICAM‐1 and VCAM‐1 expression by HGF was inhibited by nuclear factor‐kappaB (NF‐κB) activation inhibitor (MG‐132). Mitogen‐activated protein kinases (MAPK) inhibitors did not influence ICAM‐1 expression induced by TNF‐α. Interestingly, VCAM‐1 expression was enhanced by MEK inhibitor (PD98059) and c‐Jun NH2‐terminal kinase (JNK) inhibitor (SP600125). These results mean that the balance of cytokines in periodontally diseased tissue may be essential for control of ICAM‐1 and VCAM‐1 expression on HGF, and the balance of ICAM‐1 and VCAM‐1 expression might be important for regulation of leucocytes infiltration and retention in periodontally diseased tissue.