Identification of a New HLA-B Allele (B1576) by Haplotype Specific Extraction

Abstract Routine typing of a potential bone marrow donor by sequence-specific oligonucleotide probes (SSOP) and sequence based typing (SBT) produced inconclusive subtyping results, suggesting a new allele. A magnetic bead-based method, haplotype specific extraction (HSE), was used to separate the di...

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Bibliographic Details
Published in:Human immunology 2007-05, Vol.68 (5), p.418-421
Main Authors: Dapprich, Johannes, Witter, Klaus, Gabel, Harrison W, Murphy, Nancy B, Albert, Ekkehard D
Format: Article
Language:English
Subjects:
Alleles
Allergy and Immunology
Base Sequence
DNA - chemistry
DNA - genetics
DNA - isolation & purification
European Continental Ancestry Group - genetics
Haplotype specific extraction
Haplotypes - genetics
Histocompatibility Testing - methods
HLA-B Antigens - genetics
HLA-B15 Antigen
HSE
Humans
molecular haplotyping
Molecular Sequence Data
new HLA-B allele
Polymorphism, Genetic
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
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Summary:Abstract Routine typing of a potential bone marrow donor by sequence-specific oligonucleotide probes (SSOP) and sequence based typing (SBT) produced inconclusive subtyping results, suggesting a new allele. A magnetic bead-based method, haplotype specific extraction (HSE), was used to separate the diploid sample into its haploid components. The sample was then re-typed using standard SBT, revealing a new human leukocyte antigen (HLA) allele, since named B*1576. HSE used in conjunction with standard SBT is a convenient and simple tool for resolving ambiguous and novel allele combinations without the need for amplification or subcloning.
ISSN:0198-8859
1879-1166
DOI:10.1016/j.humimm.2007.01.015