Expression, purification, crystallization and preliminary X-ray analysis of YaeQ (XAC2396) from Xanthomonas axonopodis pv. citri

Xanthomonas axonopodis pv. citri YaeQ (XAC2396) is a member of a family of bacterial proteins conserved in several Gram‐negative pathogens. Here, the cloning, expression, purification and crystallization of the 182‐residue (20.6 kDa) YaeQ protein are described. Recombinant YaeQ containing selenometh...

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Bibliographic Details
Published in:Acta crystallographica. Section F, Structural biology and crystallization communications Structural biology and crystallization communications, 2005-05, Vol.61 (5), p.493-495
Main Authors: Guzzo, Cristiane R., Nagem, Ronaldo A. P., Galvão-Botton, Leonor M. P., Guimarães, Beatriz G., Medrano, Francisco J., Barbosa, João A. R. G., Farah, Chuck S.
Format: Article
Language:English
Subjects:
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - isolation & purification
Bacterial Proteins - metabolism
Cloning, Molecular
CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY
CRYSTALLIZATION
Crystallization Communications
Crystallography, X-Ray
CRYSTALS
DENSITY
DIFFRACTION
DISPERSIONS
ELECTRON DENSITY
MOLECULES
PROTEINS
Recombinant Proteins - chemistry
Recombinant Proteins - genetics
Recombinant Proteins - isolation & purification
Recombinant Proteins - metabolism
RESOLUTION
Selenomethionine - chemistry
Selenomethionine - metabolism
SPACE GROUPS
WAVELENGTHS
XAC2396
Xanthomonas - enzymology
Xanthomonas - genetics
Xanthomonas - metabolism
YaeQ
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Summary:Xanthomonas axonopodis pv. citri YaeQ (XAC2396) is a member of a family of bacterial proteins conserved in several Gram‐negative pathogens. Here, the cloning, expression, purification and crystallization of the 182‐residue (20.6 kDa) YaeQ protein are described. Recombinant YaeQ containing selenomethionine was crystallized in space group P21 and crystals diffracted to 1.9 Å resolution at a synchrotron source. The unit‐cell parameters are a = 39.75, b = 91.88, c = 48.03 Å, β = 108.37°. The calculated Matthews coefficient suggests the presence of two YaeQ molecules in the asymmetric unit. Initial experimental phases were calculated by the multiple‐wavelength anomalous dispersion technique and an interpretable electron‐density map was obtained.
ISSN:1744-3091
1744-3091
DOI:10.1107/S1744309105010985