The sarcoglycan–sarcospan complex localization in mouse retina is independent from dystrophins

• Published in: Neuroscience research 2005-09, Vol.53 (1), p.25-33
• Main Authors: Fort, Patrice, Estrada, Francisco-Javier, Bordais, Agnès, Mornet, Dominique, Sahel, José-Alain, Picaud, Serge, Vargas, Haydeé Rosas, Coral-Vázquez, Ramón M., Rendon, Alvaro
• Format: Article
• Language: English
• Subjects: Animals; Carrier Proteins; Carrier Proteins - metabolism; Cells, Cultured; Cellular Biology; Dystrophin; Dystrophin - metabolism; Dystrophins; Gene Expression Regulation; Gene Expression Regulation - physiology; Glutamate Synthase; Glutamate Synthase - metabolism; Immunohistochemistry; Immunohistochemistry - methods; Life Sciences; Macromolecular Substances; Macromolecular Substances - metabolism; mdx3cv; Membrane Proteins; Membrane Proteins - metabolism; Mice; Mice, Inbred C57BL; Mice, Inbred mdx; Mouse; Neoplasm Proteins; Neoplasm Proteins - metabolism; Neurofilament Protei; Neurofilament Proteins - metabolism; Neuroglia - metabolism; Retina; Retina - cytology; Retina - metabolism; Retinal Ganglion Cells - metabolism; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA, Messenger - biosynthesis; Sarcoglycans; Sarcoglycans - metabolism; Sarcospan
• ISSN: 0168-0102; 1872-8111
• DOI: 10.1016/j.neures.2005.05.007

The sarcoglycan–sarcospan (SG–SSPN) complex is part of the dystrophin–glycoprotein complex that has been extensively characterized in muscle. To establish the framework for functional studies of sarcoglycans in retina here, we quantified sarcoglycans mRNA levels with real-time reverse transcriptase-polymerase chain reaction (RT-PCR) and performed immunohistochemistry to determine their cellular and subcellular distribution. We showed that the β-, δ-, γ-, ɛ-sarcoglycans and sarcospan are expressed in mouse retina. They are localized predominantly in the outer and the inner limiting membranes, probably in the Müller cells and also in the ganglion cells axons where the expression of dystrophins have never been reported. We also investigated the status of the sarcoglycans in the retina of mdx 3cv mutant mice for all Duchene Muscular Dystrophy (DMD) gene products. The absence of dystrophin did not produce any change in the sarcoglycan–sarcospan components expression and distribution.