Human papillomavirus in normal conjunctival tissue and in conjunctival papilloma: types and frequencies in a large series

Aim: To examine conjunctival papilloma and normal conjunctival tissue for the presence of human papillomavirus (HPV). Methods: Archival paraffin wax-embedded tissue from 165 conjunctival papillomas and from 20 histological normal conjunctival biopsy specimens was analysed for the presence of HPV by...

Full description

Saved in:
Bibliographic Details
Published in:British journal of ophthalmology 2007-08, Vol.91 (8), p.1014-1015
Main Authors: Sjö, Nicolai Christian, von Buchwald, Christian, Cassonnet, Patricia, Norrild, Bodil, Prause, Jan Ulrik, Vinding, Troels, Heegaard, Steffen
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Biological and medical sciences
Biomedical research
Biopsy
Cervical cancer
Clinical Science - Scientific Report
Conjunctiva
Conjunctiva - pathology
Conjunctival Neoplasms
Conjunctival Neoplasms - pathology
Conjunctival Neoplasms - virology
Deoxyribonucleic acid
DNA
HPV
Human papillomavirus
Humans
Life Sciences
Medical sciences
Middle Aged
Miscellaneous
Ophthalmology
Papilloma
Papilloma - pathology
Papilloma - virology
Papillomaviridae
Papillomaviridae - isolation & purification
Papillomavirus Infections
Papillomavirus Infections - pathology
Papillomavirus Infections - virology
Polymerase Chain Reaction
Polymerase Chain Reaction - methods
Studies
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Aim: To examine conjunctival papilloma and normal conjunctival tissue for the presence of human papillomavirus (HPV). Methods: Archival paraffin wax-embedded tissue from 165 conjunctival papillomas and from 20 histological normal conjunctival biopsy specimens was analysed for the presence of HPV by PCR. Specimens considered HPV positive using consensus primers, but with a negative or uncertain PCR result using type-specific HPV probes, were analysed with DNA sequencing. Results: HPV was present in 86 of 106 (81%) β-globin-positive papillomas. HPV type 6 was positive in 80 cases, HPV type 11 was identified in 5 cases and HPV type 45 was present in a single papilloma. All the 20 normal conjunctival biopsy specimens were β-globin positive and HPV negative. Conclusion: There is a strong association between HPV and conjunctival papilloma. The study presents the largest material of conjunctival papilloma investigated for HPV and the first investigation of HPV in normal conjunctival tissue. HPV types 6 and 11 are the most common HPV types in conjunctival papilloma. This also is the first report of HPV type 45 in conjunctival papilloma.
ISSN:0007-1161
1468-2079
DOI:10.1136/bjo.2006.108811