Evidence for field change in oral cancer based on cytokeratin expression

It was hypothesised that one may be able to visualise field changes, which are proposed to exist around tumours, as alterations in keratin intermediate filament protein expression. Standard immunohistochemical analysis using a panel of monoclonal anti-keratin antibodies was applied to fresh tissue s...

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Bibliographic Details
Published in:British journal of cancer 1993-06, Vol.67 (6), p.1324-1330
Main Authors: Ogden, GR, Lane, EB, Hopwood, DV, Chisholm, DM
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Cancer Research
Carcinoma, Squamous Cell - chemistry
Carcinoma, Squamous Cell - pathology
Cytoskeleton - chemistry
Drug Resistance
Ent. Stomatology
Epidemiology
experimental-therapeutics
Female
Humans
Immunohistochemistry
Investigative techniques, diagnostic techniques (general aspects)
Keratins - analysis
Keratins - physiology
Male
Medical sciences
Middle Aged
Molecular Medicine
Mouth - chemistry
Mouth Neoplasms - chemistry
Mouth Neoplasms - pathology
Mucous Membrane - chemistry
Oncology
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
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Summary:It was hypothesised that one may be able to visualise field changes, which are proposed to exist around tumours, as alterations in keratin intermediate filament protein expression. Standard immunohistochemical analysis using a panel of monoclonal anti-keratin antibodies was applied to fresh tissue sections to look for subtle changes in epithelial differentiation not visible in H&E sections. Such changes were observed in clinically normal epithelium from oral cancer patients, involving primarily substantial expression of keratins K8/K7 (using CAM 5.2) in the basal cells of 12 out of 34 biopsies, and also a trend towards a reduction in the complexity of keratin differentiation. Monitoring such changes may prove to be a valuable adjunct to conventional H&E staining if found to have prognostic and diagnostic significance.
ISSN:0007-0920
1532-1827
DOI:10.1038/bjc.1993.245