Genome scanning of breast cancers by two-dimensional DNA typing

We have recently used two-dimensional DNA typing to detect genetic alterations in breast tumours. This method, which is based on size separation in neutral gels and sequence separation in denaturing gradient gels followed by hybridisation analysis with mini- and microsatellite core probes, allows th...

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Bibliographic Details
Published in:British journal of cancer 1994-01, Vol.69 (1), p.84-92
Main Authors: Verwest, AM, de Leeuw, WJF, Molijn, AC, Andersen, TI, Börresen, A-L, Mullaart, E, Uitterlinden, AG, Vijg, J
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Biomarkers, Tumor - analysis
Biomedical and Life Sciences
Biomedicine
Blotting, Southern
breast
Breast Neoplasms - blood
Breast Neoplasms - genetics
Cancer Research
carcinoma
Carcinoma, Ductal, Breast - blood
Carcinoma, Ductal, Breast - genetics
DNA
DNA - analysis
DNA Probes
DNA, Neoplasm - analysis
DNA, Neoplasm - blood
DNA, Neoplasm - genetics
Drug Resistance
Electrophoresis, Gel, Two-Dimensional
Epidemiology
experimental-oncology
Female
Genome, Human
genomes
Gynecology. Andrology. Obstetrics
Humans
Mammary gland diseases
man
Medical sciences
Molecular Medicine
Oncology
Predictive Value of Tests
Prognosis
scanning
Tumors
use
Citations: Items that cite this one
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Description
Summary:We have recently used two-dimensional DNA typing to detect genetic alterations in breast tumours. This method, which is based on size separation in neutral gels and sequence separation in denaturing gradient gels followed by hybridisation analysis with mini- and microsatellite core probes, allows the simultaneous analysis of hundreds of allelic fragments in a very short time. Here we demonstrate the potency of this method for total genome scanning of the tumour genome by analysing a small series of breast cancers. Comparison of tumour and normal DNA from ten breast cancer patients, using two-dimensional DNA typing with four core probes, revealed a considerable number of genomic alterations. In contrast, with Southern blot analysis only a few alterations were observed using the same probes. Most of the changes observed (74%) were deletions (absence of spots in the tumour) while 20% corresponded to amplifications (spots of higher intensity in the tumour) and 5% were new spots (gains). About 10% of the genomic changes detected appeared to occur in the tumours of more than one patient.
ISSN:0007-0920
1532-1827
DOI:10.1038/bjc.1994.13