Intratumoral induction of tumour necrosis factor by systemic administration of Bordetella pertussis vaccine

• Published in: British journal of cancer 1990-09, Vol.62 (3), p.372-375
• Main Authors: Minagawa, H, Kobayashi, H, Yoshida, H, Teranishi, M, Morikawa, A, Abe, S, Oshima, H, Mizuno, D-I
• Format: Article
• Language: English
• Subjects: Animals; Antibodies - pharmacology; Antineoplastic agents; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Cancer Research; Drug Administration Routes; Drug Resistance; Epidemiology; experimental-oncology; Female; Fibrosarcoma - drug therapy; Immunotherapy; Macrophages - immunology; Medical sciences; Mice; Mice, Inbred Strains; Molecular Medicine; Neoplasms, Experimental - drug therapy; Neoplasms, Experimental - metabolism; Oncology; Pertussis Vaccine - administration & dosage; Pertussis Vaccine - pharmacology; Pharmacology. Drug treatments; Picibanil - administration & dosage; Picibanil - pharmacology; Time Factors; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 0007-0920; 1532-1827
• DOI: 10.1038/bjc.1990.300

Intratumoral induction of tumour necrosis factor (TNF) by administration of Bordetella pertussis vaccine (BPV) as compared with that by the agent OK-432 was investigated in mice. Two hours after such administration tumour tissues tested were resected from the mice, homogenised, and the TNF activities in the homogenate were assayed using a L-929 fibroblast assay. Intravenous injection of BPV into mice bearing the MM46 carcinoma resulted in a greater concentration of TNF in the tumour homogenate than in the serum. With OK-432, however, there was a greater concentration of TNF in the serum than in the tumour homogenates. A high level of intratumoral TNF induction by BPV was also observed in mice bearing Meth A fibrosarcoma or Lewis lung carcinoma. The therapeutic effect against the Meth A fibrosarcoma was in parallel with the intratumoral TNF activity. Intratumoral TNF activity is therefore believed to be a good index of therapeutic effect.