Studies on interleukin 2 receptor expression and IL-2 production by murine T cell lymphomas

In order to study the possible role of the T-lymphocyte growth factor, Interleukin 2 (IL-2), and/or of the IL-2 receptor in the autonomous growth of leukaemic cells, 15 mouse leukaemic cell lines of various aetiology were analyzed for (i) IL-2 receptor expression and (ii) for the capacity to secrete...

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Bibliographic Details
Published in:British journal of cancer 1985-01, Vol.51 (1), p.23-30
Main Authors: Diamantstein, T, Osawa, H, Graf, L, Schirrmacher, V
Format: Article
Language:English
Subjects:
Animal tumors. Experimental tumors
Animals
Antibodies, Monoclonal - immunology
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Cancer Research
Cell Division
Cell Line
Drug Resistance
Epidemiology
Experimental malignant blood diseases
interleukin 2
Interleukin-2 - biosynthesis
Leukemia, Experimental - immunology
Leukemia, Experimental - metabolism
lymphocytes T
lymphoma
Medical sciences
Mice
Mice, Inbred Strains
Molecular Medicine
Oncology
original-article
receptors
Receptors, Immunologic - analysis
Receptors, Immunologic - immunology
Receptors, Interleukin-2
T-Lymphocytes - pathology
Tumors
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Summary:In order to study the possible role of the T-lymphocyte growth factor, Interleukin 2 (IL-2), and/or of the IL-2 receptor in the autonomous growth of leukaemic cells, 15 mouse leukaemic cell lines of various aetiology were analyzed for (i) IL-2 receptor expression and (ii) for the capacity to secrete IL-2. Several but not all of the cell lines tested were IL-2 receptor positive. The cells constitutively expressing IL-2 receptors at their surface could not be stimulated to secrete IL-2. Cell producing and secreting IL-2 did not express detectable amounts of IL-2 receptors at their surface. It has been demonstrated that proliferation of the leukaemic cells was independent of exogenous IL-2. The monoclonal anti-IL-2 receptor antibody AMT-13 inhibited IL-2 dependent proliferation of activated normal T-lymphocytes but failed to inhibit the growth of IL-2 receptor expressing leukaemic cells. The results argue against the autocrine stimulation hypothesis but do not exclude the possibility of involvement of functionally altered IL-2 receptors on autonomous cell growth.
ISSN:0007-0920
1532-1827
DOI:10.1038/bjc.1985.4