The effect of respiratory muscle training with CO2 breathing on cellular adaptation of mdx mouse diaphragm

The aim of our study was to investigate the cellular mechanisms induced by hypercapnic stimulation of ventilation, during 6 weeks/30 min per day, in 10 mdx and 8 C57BL10 mice (10+/-0.2 months old). Ten mdx and eight C57BL10 mice served as control group. This respiratory training increases in vitro m...

Full description

Saved in:
Bibliographic Details
Published in:Neuromuscular disorders : NMD 2005-06, Vol.15 (6), p.427-436
Main Authors: Matecki, Stefan, Rivier, François, Hugon, Gerald, Koechlin, Christelle, Michel, Alain, Prefaut, Christian, Mornet, Dominique, Ramonatxo, Michele
Format: Article
Language:English
Subjects:
Adaptation, Physiological
Animals
Blotting, Western
Body Weight
Breathing Exercises
Carbon Dioxide
Carbon Dioxide - pharmacology
Cellular Biology
Citrate (si)-Synthase
Citrate (si)-Synthase - metabolism
Diaphragm
Diaphragm - cytology
Diaphragm - physiology
Dystrophin
Dystrophin - metabolism
Dystrophin-Associated Proteins
Dystrophin-Associated Proteins - metabolism
Female
Hematoxylin
Hypercapnia
Hypercapnia - physiopathology
Hyperventilation
Hyperventilation - physiopathology
Isometric Contractio
Isometric Contraction - physiology
Life Sciences
Male
Mice
Mice, Inbred C57BL
Mice, Inbred mdx
Muscle Fibers, Skeletal - metabolism
Muscular Dystrophy, Animal - physiopathology
Muscular Dystrophy, Animal - therapy
Organ Size
Respiratory Mechanics - physiology
Utrophin - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of our study was to investigate the cellular mechanisms induced by hypercapnic stimulation of ventilation, during 6 weeks/30 min per day, in 10 mdx and 8 C57BL10 mice (10+/-0.2 months old). Ten mdx and eight C57BL10 mice served as control group. This respiratory training increases in vitro maximal tetanic tension of the diaphragm only in mdx mice. Western blot analysis of diaphragm showed: (1) an over-expression of alpha-dystrobrevin in mdx and C57BL10 training group compared to control group (8100+/-710 versus 6100+/-520 and 2800+/-400 versus 2200+/-250 arbitrary units); (2) a decrease in utrophin expression only in mdx training group compared to control group (2100+/-320 versus 3100+/-125 arbitrary units). Daily respiratory muscle training in mdx mice, induces a beneficial effect on diaphragm strength, with an over-expression of alpha-dystrobrevin. Further studies are needed to determine if, in absence of dystrophin, the over-expression of alpha-dystrobrevin could be interpreted as a possible pathway to improve function of dystrophic muscle.
ISSN:0960-8966
DOI:10.1016/j.nmd.2005.01.014