Calcium-mediated, purinergic stimulation and polarized localization of calcium-sensitive adenylyl cyclase isoforms in human airway epithelia

Purinergic stimulation of human airway epithelia results in a prolonged increase in ciliary beat frequency that depends on calcium-mediated cAMP production [Lieb, T., Wijkstrom Frei, C., Frohock, J.I., Bookman, R.J. and Salathe, M. (2002) Prolonged increase in ciliary beat frequency after short-term...

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Bibliographic Details
Published in:FEBS letters 2007-07, Vol.581 (17), p.3241-3246
Main Authors: Nlend, Marie-Christine, Schmid, Andreas, Sutto, Zoltan, Ransford, George A., Conner, Gregory E., Fregien, Nevis, Salathe, Matthias
Format: Article
Language:English
Subjects:
Adenylyl cyclase isoforms
Adenylyl Cyclases - genetics
Adenylyl Cyclases - metabolism
Airway epithelium
air–liquid interface
ALI
Calcium - pharmacology
cAMP production
CBF
Cell Polarity - physiology
Cells, Cultured
ciliary beat frequency
Ciliary beating
Cyclic AMP - metabolism
Epithelial Cells - drug effects
Epithelial Cells - enzymology
Epithelial Cells - metabolism
Fluorescent Antibody Technique
Humans
Isoenzymes - genetics
Isoenzymes - metabolism
Polarized expression
Purines - pharmacology
Respiratory Mucosa - drug effects
Respiratory Mucosa - enzymology
Respiratory Mucosa - metabolism
Reverse Transcriptase Polymerase Chain Reaction
reverse transcription polymerase chain reaction
RT-PCR
Tissue Distribution
tmACs
transmembrane adenylyl cyclases
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Summary:Purinergic stimulation of human airway epithelia results in a prolonged increase in ciliary beat frequency that depends on calcium-mediated cAMP production [Lieb, T., Wijkstrom Frei, C., Frohock, J.I., Bookman, R.J. and Salathe, M. (2002) Prolonged increase in ciliary beat frequency after short-term purinergic stimulation in human airway epithelial cells. J. Physiol. (Lond.) 538, 633–646]. Here, fully differentiated human airway epithelial cells in culture are shown to express calcium-stimulated transmembrane adenylyl cyclase (tmAC) isoforms (types 1, 3, and 8) by reverse transcription polymerase chain reaction. Immunohistochemistry of tracheal sections and fully differentiated airway epithelial cell cultures revealed polarized expression of these tmACs, with types 1 and 8 localized to the apical membrane and thus at the position required for ciliary regulation. Real-time, ciliated-cell specific cAMP production by tmACs upon apical, purinergic stimulation with UTP was confirmed using fluorescent energy resonance transfer between fluorescently tagged PKA subunits.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2007.06.015