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Interaction between the inhibitory action of acetylcholine and the α‐adrenoceptor autoinhibitory feedback system on release of [3H]‐noradrenaline from rat atria and rabbit ear artery
1 Stimulation‐induced increases in the efflux of radioactivity (S‐I efflux) were measured in the bathing medium following labelling of the noradrenergic transmitter pools of rat atria and rabbit artery preparations with [3H]‐noradrenaline. 2 In atria stimulated with trains of 16 or 60 pulses at 2 Hz...
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| Published in: | British journal of pharmacology 1985-03, Vol.84 (3), p.697-705 |
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| Language: | English |
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| container_issue | 3 |
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| container_title | British journal of pharmacology |
| container_volume | 84 |
| creator | Loiacono, R.E. Rand, M.J. Story, D.F. |
| description | 1
Stimulation‐induced increases in the efflux of radioactivity (S‐I efflux) were measured in the bathing medium following labelling of the noradrenergic transmitter pools of rat atria and rabbit artery preparations with [3H]‐noradrenaline.
2
In atria stimulated with trains of 16 or 60 pulses at 2 Hz, phentolamine enhanced, whereas acetylcholine inhibited S‐I efflux. With trains of 16 pulses phentolamine had a smaller enhancing effect than with trains of 60 pulses, whereas the inhibitory effect of acetylcholine was more pronounced with 16 pulses of stimulation.
3
The inhibitory effect of acetylcholine was markedly enhanced by phentolamine when stimulation was with 60 pulses. With 16 pulses of stimulation the effect of acetylcholine was unaltered by phentolamine and abolished by the α2‐adrenoceptor agonist 3,4‐dihydroxyphenylimino‐2‐imidazolidine (DPI).
4
Phentolamine had no effect on the negative inotropic effect of acetylcholine on driven left atrial preparations.
5
In arterial preparations stimulated with trains of 30 pulses at 1 Hz, both acetylcholine and clonidine inhibited S‐I efflux, whereas yohimbine and idazoxan enhanced S‐I efflux. Combining acetylcholine with clonidine did not alter the inhibitory effect of clonidine but the combination of acetylcholine with yohimbine or idazoxan abolished the marked enhancing effects of yohimbine or idazoxan on S‐I efflux.
6
These findings indicate that there may be a reciprocal interaction between prejunctional α‐adrenoceptors and prejunctional muscarinic cholinoceptors. |
| doi_str_mv | 10.1111/j.1476-5381.1985.tb16152.x |
| format | article |
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Stimulation‐induced increases in the efflux of radioactivity (S‐I efflux) were measured in the bathing medium following labelling of the noradrenergic transmitter pools of rat atria and rabbit artery preparations with [3H]‐noradrenaline.
2
In atria stimulated with trains of 16 or 60 pulses at 2 Hz, phentolamine enhanced, whereas acetylcholine inhibited S‐I efflux. With trains of 16 pulses phentolamine had a smaller enhancing effect than with trains of 60 pulses, whereas the inhibitory effect of acetylcholine was more pronounced with 16 pulses of stimulation.
3
The inhibitory effect of acetylcholine was markedly enhanced by phentolamine when stimulation was with 60 pulses. With 16 pulses of stimulation the effect of acetylcholine was unaltered by phentolamine and abolished by the α2‐adrenoceptor agonist 3,4‐dihydroxyphenylimino‐2‐imidazolidine (DPI).
4
Phentolamine had no effect on the negative inotropic effect of acetylcholine on driven left atrial preparations.
5
In arterial preparations stimulated with trains of 30 pulses at 1 Hz, both acetylcholine and clonidine inhibited S‐I efflux, whereas yohimbine and idazoxan enhanced S‐I efflux. Combining acetylcholine with clonidine did not alter the inhibitory effect of clonidine but the combination of acetylcholine with yohimbine or idazoxan abolished the marked enhancing effects of yohimbine or idazoxan on S‐I efflux.
6
These findings indicate that there may be a reciprocal interaction between prejunctional α‐adrenoceptors and prejunctional muscarinic cholinoceptors.</description><identifier>ISSN: 0007-1188</identifier><identifier>EISSN: 1476-5381</identifier><identifier>DOI: 10.1111/j.1476-5381.1985.tb16152.x</identifier><identifier>PMID: 2859064</identifier><identifier>CODEN: BJPCBM</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Acetylcholine - pharmacology ; Adrenergic alpha-Antagonists - pharmacology ; Animals ; Arteries - metabolism ; Biological and medical sciences ; Catecholamines - pharmacology ; Clonidine - pharmacology ; Dioxanes - pharmacology ; Ear - blood supply ; Electric Stimulation ; Feedback ; Female ; Fundamental and applied biological sciences. Psychology ; Heart Atria - metabolism ; Idazoxan ; Imidazolines ; In Vitro Techniques ; Male ; Muscle, Smooth, Vascular - innervation ; Muscle, Smooth, Vascular - metabolism ; Myocardial Contraction - drug effects ; Myocardium - metabolism ; Norepinephrine - metabolism ; Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ ; Phentolamine - pharmacology ; Rabbits ; Rats ; Rats, Inbred Strains ; Receptors, Adrenergic, alpha - drug effects ; Vertebrates: nervous system and sense organs ; Yohimbine - pharmacology</subject><ispartof>British journal of pharmacology, 1985-03, Vol.84 (3), p.697-705</ispartof><rights>1985 British Pharmacological Society</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5073-591dbcf0bc2de1fb263d73a17bf469fe8e5c34f86c3c7ccb0a59f805957d37d43</citedby><cites>FETCH-LOGICAL-c5073-591dbcf0bc2de1fb263d73a17bf469fe8e5c34f86c3c7ccb0a59f805957d37d43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1987138/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1987138/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8481322$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2859064$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Loiacono, R.E.</creatorcontrib><creatorcontrib>Rand, M.J.</creatorcontrib><creatorcontrib>Story, D.F.</creatorcontrib><title>Interaction between the inhibitory action of acetylcholine and the α‐adrenoceptor autoinhibitory feedback system on release of [3H]‐noradrenaline from rat atria and rabbit ear artery</title><title>British journal of pharmacology</title><addtitle>Br J Pharmacol</addtitle><description>1
Stimulation‐induced increases in the efflux of radioactivity (S‐I efflux) were measured in the bathing medium following labelling of the noradrenergic transmitter pools of rat atria and rabbit artery preparations with [3H]‐noradrenaline.
2
In atria stimulated with trains of 16 or 60 pulses at 2 Hz, phentolamine enhanced, whereas acetylcholine inhibited S‐I efflux. With trains of 16 pulses phentolamine had a smaller enhancing effect than with trains of 60 pulses, whereas the inhibitory effect of acetylcholine was more pronounced with 16 pulses of stimulation.
3
The inhibitory effect of acetylcholine was markedly enhanced by phentolamine when stimulation was with 60 pulses. With 16 pulses of stimulation the effect of acetylcholine was unaltered by phentolamine and abolished by the α2‐adrenoceptor agonist 3,4‐dihydroxyphenylimino‐2‐imidazolidine (DPI).
4
Phentolamine had no effect on the negative inotropic effect of acetylcholine on driven left atrial preparations.
5
In arterial preparations stimulated with trains of 30 pulses at 1 Hz, both acetylcholine and clonidine inhibited S‐I efflux, whereas yohimbine and idazoxan enhanced S‐I efflux. Combining acetylcholine with clonidine did not alter the inhibitory effect of clonidine but the combination of acetylcholine with yohimbine or idazoxan abolished the marked enhancing effects of yohimbine or idazoxan on S‐I efflux.
6
These findings indicate that there may be a reciprocal interaction between prejunctional α‐adrenoceptors and prejunctional muscarinic cholinoceptors.</description><subject>Acetylcholine - pharmacology</subject><subject>Adrenergic alpha-Antagonists - pharmacology</subject><subject>Animals</subject><subject>Arteries - metabolism</subject><subject>Biological and medical sciences</subject><subject>Catecholamines - pharmacology</subject><subject>Clonidine - pharmacology</subject><subject>Dioxanes - pharmacology</subject><subject>Ear - blood supply</subject><subject>Electric Stimulation</subject><subject>Feedback</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Heart Atria - metabolism</subject><subject>Idazoxan</subject><subject>Imidazolines</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Muscle, Smooth, Vascular - innervation</subject><subject>Muscle, Smooth, Vascular - metabolism</subject><subject>Myocardial Contraction - drug effects</subject><subject>Myocardium - metabolism</subject><subject>Norepinephrine - metabolism</subject><subject>Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ</subject><subject>Phentolamine - pharmacology</subject><subject>Rabbits</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Receptors, Adrenergic, alpha - drug effects</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>Yohimbine - pharmacology</subject><issn>0007-1188</issn><issn>1476-5381</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><recordid>eNqVkUGO0zAUhiMEGsrAEZAihNil2HEcOywQMAI60kiwgBVClu08U5fU7tguM9lxBK7CmjtwCE6C20bVsMQbW_r_9_lJX1E8wmiO83m6muOGtRUlHM9xx-k8KdxiWs-vbxWzY3S7mCGEWIUx53eLezGuEMohoyfFSc1ph9pmVvw6dwmC1Ml6VypIVwCuTEsorVtaZZMPYzml3uQXpHHQSz9YB6V0_b76--ef7z9kH8B5DZs8Uspt8jcABqBXUn8t4xgTrMsMCzCAjLCDfiKLzxngfNgz5J5tgl-XQaZSpmDl_qsgVeaVIDM_5KXH-8UdI4cID6b7tPj45vWHs0V18e7t-dnLi0pTxEhFO9wrbZDSdQ_YqLolPSMSM2WatjPAgWrSGN5qopnWCknaGY5oR1lPWN-Q0-L5gbvZqjX0GlwKchCbYNcyjMJLK_5NnF2KL_6byG4YJjwDnkyA4C-3EJNY26hhGKQDv42CtdlFy7tcfHYo6uBjDGCOn2AkdurFSuz8ip3fHZ6KSb24zsMPb655HJ1c5_zxlMuo5WCCdNrGY403HJO6zrUXh9qVHWD8jwXEq_cL3HaMkL-uOdW1</recordid><startdate>198503</startdate><enddate>198503</enddate><creator>Loiacono, R.E.</creator><creator>Rand, M.J.</creator><creator>Story, D.F.</creator><general>Blackwell Publishing Ltd</general><general>Nature Publishing</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>198503</creationdate><title>Interaction between the inhibitory action of acetylcholine and the α‐adrenoceptor autoinhibitory feedback system on release of [3H]‐noradrenaline from rat atria and rabbit ear artery</title><author>Loiacono, R.E. ; Rand, M.J. ; Story, D.F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5073-591dbcf0bc2de1fb263d73a17bf469fe8e5c34f86c3c7ccb0a59f805957d37d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Acetylcholine - pharmacology</topic><topic>Adrenergic alpha-Antagonists - pharmacology</topic><topic>Animals</topic><topic>Arteries - metabolism</topic><topic>Biological and medical sciences</topic><topic>Catecholamines - pharmacology</topic><topic>Clonidine - pharmacology</topic><topic>Dioxanes - pharmacology</topic><topic>Ear - blood supply</topic><topic>Electric Stimulation</topic><topic>Feedback</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Heart Atria - metabolism</topic><topic>Idazoxan</topic><topic>Imidazolines</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Muscle, Smooth, Vascular - innervation</topic><topic>Muscle, Smooth, Vascular - metabolism</topic><topic>Myocardial Contraction - drug effects</topic><topic>Myocardium - metabolism</topic><topic>Norepinephrine - metabolism</topic><topic>Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ</topic><topic>Phentolamine - pharmacology</topic><topic>Rabbits</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Receptors, Adrenergic, alpha - drug effects</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>Yohimbine - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Loiacono, R.E.</creatorcontrib><creatorcontrib>Rand, M.J.</creatorcontrib><creatorcontrib>Story, D.F.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Loiacono, R.E.</au><au>Rand, M.J.</au><au>Story, D.F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interaction between the inhibitory action of acetylcholine and the α‐adrenoceptor autoinhibitory feedback system on release of [3H]‐noradrenaline from rat atria and rabbit ear artery</atitle><jtitle>British journal of pharmacology</jtitle><addtitle>Br J Pharmacol</addtitle><date>1985-03</date><risdate>1985</risdate><volume>84</volume><issue>3</issue><spage>697</spage><epage>705</epage><pages>697-705</pages><issn>0007-1188</issn><eissn>1476-5381</eissn><coden>BJPCBM</coden><abstract>1
Stimulation‐induced increases in the efflux of radioactivity (S‐I efflux) were measured in the bathing medium following labelling of the noradrenergic transmitter pools of rat atria and rabbit artery preparations with [3H]‐noradrenaline.
2
In atria stimulated with trains of 16 or 60 pulses at 2 Hz, phentolamine enhanced, whereas acetylcholine inhibited S‐I efflux. With trains of 16 pulses phentolamine had a smaller enhancing effect than with trains of 60 pulses, whereas the inhibitory effect of acetylcholine was more pronounced with 16 pulses of stimulation.
3
The inhibitory effect of acetylcholine was markedly enhanced by phentolamine when stimulation was with 60 pulses. With 16 pulses of stimulation the effect of acetylcholine was unaltered by phentolamine and abolished by the α2‐adrenoceptor agonist 3,4‐dihydroxyphenylimino‐2‐imidazolidine (DPI).
4
Phentolamine had no effect on the negative inotropic effect of acetylcholine on driven left atrial preparations.
5
In arterial preparations stimulated with trains of 30 pulses at 1 Hz, both acetylcholine and clonidine inhibited S‐I efflux, whereas yohimbine and idazoxan enhanced S‐I efflux. Combining acetylcholine with clonidine did not alter the inhibitory effect of clonidine but the combination of acetylcholine with yohimbine or idazoxan abolished the marked enhancing effects of yohimbine or idazoxan on S‐I efflux.
6
These findings indicate that there may be a reciprocal interaction between prejunctional α‐adrenoceptors and prejunctional muscarinic cholinoceptors.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>2859064</pmid><doi>10.1111/j.1476-5381.1985.tb16152.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | British journal of pharmacology, 1985-03, Vol.84 (3), p.697-705 |
| issn | 0007-1188 1476-5381 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1987138 |
| source | PubMed Central |
| subjects | Acetylcholine - pharmacology Adrenergic alpha-Antagonists - pharmacology Animals Arteries - metabolism Biological and medical sciences Catecholamines - pharmacology Clonidine - pharmacology Dioxanes - pharmacology Ear - blood supply Electric Stimulation Feedback Female Fundamental and applied biological sciences. Psychology Heart Atria - metabolism Idazoxan Imidazolines In Vitro Techniques Male Muscle, Smooth, Vascular - innervation Muscle, Smooth, Vascular - metabolism Myocardial Contraction - drug effects Myocardium - metabolism Norepinephrine - metabolism Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ Phentolamine - pharmacology Rabbits Rats Rats, Inbred Strains Receptors, Adrenergic, alpha - drug effects Vertebrates: nervous system and sense organs Yohimbine - pharmacology |
| title | Interaction between the inhibitory action of acetylcholine and the α‐adrenoceptor autoinhibitory feedback system on release of [3H]‐noradrenaline from rat atria and rabbit ear artery |
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