Loading…
Up-regulation of the Ku heterodimer in Drosophila testicular cyst cells
In Drosophila, developing germline cysts in testis are enveloped by two somatic cyst cells essential for germline development and male reproduction. The cyst cells continue development along with the germline. However, the mechanisms of somatic gene expression in testes are poorly understood. We rep...
Saved in:
| Published in: | FEBS letters 2007-05, Vol.581 (9), p.1707-1715 |
|---|---|
| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c5618-1337985195a444404b4afdc874f4cba384fd6180d365d4e8dbad9ee0c4d55db33 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c5618-1337985195a444404b4afdc874f4cba384fd6180d365d4e8dbad9ee0c4d55db33 |
| container_end_page | 1715 |
| container_issue | 9 |
| container_start_page | 1707 |
| container_title | FEBS letters |
| container_volume | 581 |
| creator | Boutanaev, Alexander M. Mikhaylova, Lyudmila M. Nurminsky, Dmitry I. |
| description | In
Drosophila, developing germline cysts in testis are enveloped by two somatic cyst cells essential for germline development and male reproduction. The cyst cells continue development along with the germline. However, the mechanisms of somatic gene expression in testes are poorly understood. We report transcriptional up-regulation of the Ku heterodimer in cyst cells. The initial up-regulation is independent of germline, and transcription is further augmented during spermatogenesis. Abundance of Ku in the cyst cell cytoplasm suggests the role for Ku subunits in the regulation of sperm individualization. |
| doi_str_mv | 10.1016/j.febslet.2007.03.042 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1992518</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0014579307003158</els_id><sourcerecordid>19706997</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5618-1337985195a444404b4afdc874f4cba384fd6180d365d4e8dbad9ee0c4d55db33</originalsourceid><addsrcrecordid>eNqNkc1u1DAUhS0EokPhEUBZsUu4ju3E3oBo6Q-iEgvo2nLsm45HmXiwk6J5exzNCNpV8cayfM7RPfcj5C2FigJtPmyqHrs04FTVAG0FrAJePyMrKltWMt7I52QFQHkpWsVOyKuUNpDfkqqX5IS2nEpZ0xW5ut2VEe_mwUw-jEXoi2mNxbe5WOOEMTi_xVj4sfgSQwq7tR9MMWGavM2OWNh9mgqLw5Bekxe9GRK-Od6n5Pby4uf5dXnz_err-eeb0oqGypIy1iopqBKG5wO846Z3Vra857YzTPLeZR041gjHUbrOOIUIljshXMfYKfl4yN3N3RadxXGKZtC76Lcm7nUwXj_-Gf1a34V7TZWqBZU54P0xIIZfc66itz4tFcyIYU66hbwaBU8LqWqhUarNQnEQ2ryjFLH_Ow0FvbDSG31kpRdWGpjOrLLv3cMq_1xHOFlwfRD89gPu_y9VX16c1T8W8At3aAEYFUuZT4cozGzuPUadrMfRovMR7aRd8E9M-wfO8b7H</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19706997</pqid></control><display><type>article</type><title>Up-regulation of the Ku heterodimer in Drosophila testicular cyst cells</title><source>ScienceDirect Journals</source><source>Wiley-Blackwell Read & Publish Collection</source><creator>Boutanaev, Alexander M. ; Mikhaylova, Lyudmila M. ; Nurminsky, Dmitry I.</creator><creatorcontrib>Boutanaev, Alexander M. ; Mikhaylova, Lyudmila M. ; Nurminsky, Dmitry I.</creatorcontrib><description>In
Drosophila, developing germline cysts in testis are enveloped by two somatic cyst cells essential for germline development and male reproduction. The cyst cells continue development along with the germline. However, the mechanisms of somatic gene expression in testes are poorly understood. We report transcriptional up-regulation of the Ku heterodimer in cyst cells. The initial up-regulation is independent of germline, and transcription is further augmented during spermatogenesis. Abundance of Ku in the cyst cell cytoplasm suggests the role for Ku subunits in the regulation of sperm individualization.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2007.03.042</identifier><identifier>PMID: 17418821</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Animals ; Antigens, Nuclear - genetics ; Antigens, Nuclear - metabolism ; bovine serum albumin ; BSA ; Dimerization ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; double strand break ; Drosophila ; Drosophila - genetics ; Drosophila - metabolism ; DSB ; electrophoretic mobility shift assay ; EMSA ; homologous repair ; Ku Autoantigen ; Ku heterodimer ; Male ; Meiosis - genetics ; NFDM ; NHEJ ; NIB ; non-fat dry milk ; non-homologous end-joining ; nuclei isolation buffer ; PBS ; PBST ; PCR ; phosphate buffered saline ; phosphate buffered saline with 0.05% Tween-20 ; polymerase chain reaction ; Protein Subunits - genetics ; Protein Subunits - metabolism ; reverse transcription polymerase chain reaction ; RT-PCR ; SDS–PAGE ; sodium dodecyl sulfate–polyacrylamide gel electrophoresis ; Somatic cells ; Testes ; Testis - metabolism ; Transcription ; Transcription, Genetic ; Up-Regulation</subject><ispartof>FEBS letters, 2007-05, Vol.581 (9), p.1707-1715</ispartof><rights>2007 Federation of European Biochemical Societies</rights><rights>FEBS Letters 581 (2007) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5618-1337985195a444404b4afdc874f4cba384fd6180d365d4e8dbad9ee0c4d55db33</citedby><cites>FETCH-LOGICAL-c5618-1337985195a444404b4afdc874f4cba384fd6180d365d4e8dbad9ee0c4d55db33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014579307003158$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,3549,27924,27925,45780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17418821$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boutanaev, Alexander M.</creatorcontrib><creatorcontrib>Mikhaylova, Lyudmila M.</creatorcontrib><creatorcontrib>Nurminsky, Dmitry I.</creatorcontrib><title>Up-regulation of the Ku heterodimer in Drosophila testicular cyst cells</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>In
Drosophila, developing germline cysts in testis are enveloped by two somatic cyst cells essential for germline development and male reproduction. The cyst cells continue development along with the germline. However, the mechanisms of somatic gene expression in testes are poorly understood. We report transcriptional up-regulation of the Ku heterodimer in cyst cells. The initial up-regulation is independent of germline, and transcription is further augmented during spermatogenesis. Abundance of Ku in the cyst cell cytoplasm suggests the role for Ku subunits in the regulation of sperm individualization.</description><subject>Animals</subject><subject>Antigens, Nuclear - genetics</subject><subject>Antigens, Nuclear - metabolism</subject><subject>bovine serum albumin</subject><subject>BSA</subject><subject>Dimerization</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>double strand break</subject><subject>Drosophila</subject><subject>Drosophila - genetics</subject><subject>Drosophila - metabolism</subject><subject>DSB</subject><subject>electrophoretic mobility shift assay</subject><subject>EMSA</subject><subject>homologous repair</subject><subject>Ku Autoantigen</subject><subject>Ku heterodimer</subject><subject>Male</subject><subject>Meiosis - genetics</subject><subject>NFDM</subject><subject>NHEJ</subject><subject>NIB</subject><subject>non-fat dry milk</subject><subject>non-homologous end-joining</subject><subject>nuclei isolation buffer</subject><subject>PBS</subject><subject>PBST</subject><subject>PCR</subject><subject>phosphate buffered saline</subject><subject>phosphate buffered saline with 0.05% Tween-20</subject><subject>polymerase chain reaction</subject><subject>Protein Subunits - genetics</subject><subject>Protein Subunits - metabolism</subject><subject>reverse transcription polymerase chain reaction</subject><subject>RT-PCR</subject><subject>SDS–PAGE</subject><subject>sodium dodecyl sulfate–polyacrylamide gel electrophoresis</subject><subject>Somatic cells</subject><subject>Testes</subject><subject>Testis - metabolism</subject><subject>Transcription</subject><subject>Transcription, Genetic</subject><subject>Up-Regulation</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqNkc1u1DAUhS0EokPhEUBZsUu4ju3E3oBo6Q-iEgvo2nLsm45HmXiwk6J5exzNCNpV8cayfM7RPfcj5C2FigJtPmyqHrs04FTVAG0FrAJePyMrKltWMt7I52QFQHkpWsVOyKuUNpDfkqqX5IS2nEpZ0xW5ut2VEe_mwUw-jEXoi2mNxbe5WOOEMTi_xVj4sfgSQwq7tR9MMWGavM2OWNh9mgqLw5Bekxe9GRK-Od6n5Pby4uf5dXnz_err-eeb0oqGypIy1iopqBKG5wO846Z3Vra857YzTPLeZR041gjHUbrOOIUIljshXMfYKfl4yN3N3RadxXGKZtC76Lcm7nUwXj_-Gf1a34V7TZWqBZU54P0xIIZfc66itz4tFcyIYU66hbwaBU8LqWqhUarNQnEQ2ryjFLH_Ow0FvbDSG31kpRdWGpjOrLLv3cMq_1xHOFlwfRD89gPu_y9VX16c1T8W8At3aAEYFUuZT4cozGzuPUadrMfRovMR7aRd8E9M-wfO8b7H</recordid><startdate>20070501</startdate><enddate>20070501</enddate><creator>Boutanaev, Alexander M.</creator><creator>Mikhaylova, Lyudmila M.</creator><creator>Nurminsky, Dmitry I.</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20070501</creationdate><title>Up-regulation of the Ku heterodimer in Drosophila testicular cyst cells</title><author>Boutanaev, Alexander M. ; Mikhaylova, Lyudmila M. ; Nurminsky, Dmitry I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5618-1337985195a444404b4afdc874f4cba384fd6180d365d4e8dbad9ee0c4d55db33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Antigens, Nuclear - genetics</topic><topic>Antigens, Nuclear - metabolism</topic><topic>bovine serum albumin</topic><topic>BSA</topic><topic>Dimerization</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>double strand break</topic><topic>Drosophila</topic><topic>Drosophila - genetics</topic><topic>Drosophila - metabolism</topic><topic>DSB</topic><topic>electrophoretic mobility shift assay</topic><topic>EMSA</topic><topic>homologous repair</topic><topic>Ku Autoantigen</topic><topic>Ku heterodimer</topic><topic>Male</topic><topic>Meiosis - genetics</topic><topic>NFDM</topic><topic>NHEJ</topic><topic>NIB</topic><topic>non-fat dry milk</topic><topic>non-homologous end-joining</topic><topic>nuclei isolation buffer</topic><topic>PBS</topic><topic>PBST</topic><topic>PCR</topic><topic>phosphate buffered saline</topic><topic>phosphate buffered saline with 0.05% Tween-20</topic><topic>polymerase chain reaction</topic><topic>Protein Subunits - genetics</topic><topic>Protein Subunits - metabolism</topic><topic>reverse transcription polymerase chain reaction</topic><topic>RT-PCR</topic><topic>SDS–PAGE</topic><topic>sodium dodecyl sulfate–polyacrylamide gel electrophoresis</topic><topic>Somatic cells</topic><topic>Testes</topic><topic>Testis - metabolism</topic><topic>Transcription</topic><topic>Transcription, Genetic</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boutanaev, Alexander M.</creatorcontrib><creatorcontrib>Mikhaylova, Lyudmila M.</creatorcontrib><creatorcontrib>Nurminsky, Dmitry I.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boutanaev, Alexander M.</au><au>Mikhaylova, Lyudmila M.</au><au>Nurminsky, Dmitry I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Up-regulation of the Ku heterodimer in Drosophila testicular cyst cells</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2007-05-01</date><risdate>2007</risdate><volume>581</volume><issue>9</issue><spage>1707</spage><epage>1715</epage><pages>1707-1715</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>In
Drosophila, developing germline cysts in testis are enveloped by two somatic cyst cells essential for germline development and male reproduction. The cyst cells continue development along with the germline. However, the mechanisms of somatic gene expression in testes are poorly understood. We report transcriptional up-regulation of the Ku heterodimer in cyst cells. The initial up-regulation is independent of germline, and transcription is further augmented during spermatogenesis. Abundance of Ku in the cyst cell cytoplasm suggests the role for Ku subunits in the regulation of sperm individualization.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>17418821</pmid><doi>10.1016/j.febslet.2007.03.042</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 2007-05, Vol.581 (9), p.1707-1715 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1992518 |
| source | ScienceDirect Journals; Wiley-Blackwell Read & Publish Collection |
| subjects | Animals Antigens, Nuclear - genetics Antigens, Nuclear - metabolism bovine serum albumin BSA Dimerization DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism double strand break Drosophila Drosophila - genetics Drosophila - metabolism DSB electrophoretic mobility shift assay EMSA homologous repair Ku Autoantigen Ku heterodimer Male Meiosis - genetics NFDM NHEJ NIB non-fat dry milk non-homologous end-joining nuclei isolation buffer PBS PBST PCR phosphate buffered saline phosphate buffered saline with 0.05% Tween-20 polymerase chain reaction Protein Subunits - genetics Protein Subunits - metabolism reverse transcription polymerase chain reaction RT-PCR SDS–PAGE sodium dodecyl sulfate–polyacrylamide gel electrophoresis Somatic cells Testes Testis - metabolism Transcription Transcription, Genetic Up-Regulation |
| title | Up-regulation of the Ku heterodimer in Drosophila testicular cyst cells |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T03%3A31%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Up-regulation%20of%20the%20Ku%20heterodimer%20in%20Drosophila%20testicular%20cyst%20cells&rft.jtitle=FEBS%20letters&rft.au=Boutanaev,%20Alexander%20M.&rft.date=2007-05-01&rft.volume=581&rft.issue=9&rft.spage=1707&rft.epage=1715&rft.pages=1707-1715&rft.issn=0014-5793&rft.eissn=1873-3468&rft_id=info:doi/10.1016/j.febslet.2007.03.042&rft_dat=%3Cproquest_pubme%3E19706997%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c5618-1337985195a444404b4afdc874f4cba384fd6180d365d4e8dbad9ee0c4d55db33%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=19706997&rft_id=info:pmid/17418821&rfr_iscdi=true |