Profiling of molecular interactions in real time using acoustic detection

Acoustic sensors that exploit resonating quartz crystals to directly detect the binding of an analyte to a receptor are finding increasing utility in the quantification of clinically relevant analytes. We have developed a novel acoustic detection technology, which we term resonant acoustic profiling...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2007-04, Vol.22 (9), p.2382-2386
Main Authors: Godber, Benjamin, Frogley, Mark, Rehak, Marian, Sleptsov, Alexander, Thompson, Kevin S.J., Uludag, Yildiz, Cooper, Matthew A.
Format: Article
Language:English
Subjects:
Affinity
Binding
Biological and medical sciences
Biosensing Techniques
Biotechnology
Fundamental and applied biological sciences. Psychology
Kinetics
Label-free
Microfluidics
Protein Binding
Proteins - metabolism
QCM
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Summary:Acoustic sensors that exploit resonating quartz crystals to directly detect the binding of an analyte to a receptor are finding increasing utility in the quantification of clinically relevant analytes. We have developed a novel acoustic detection technology, which we term resonant acoustic profiling (RAP™). This technology builds on the fundamental basics of the “quartz crystal microbalance” or “QCM” with several key additional features including two- or four-channel automated sample delivery, in-line referencing and microfluidic sensor ‘cassettes’ that are pre-coated with easy-to-use surface chemistries. Example applications are described for the quantification of myoglobin concentration and its interaction kinetics, and for the ranking of enzyme-cofactor specificities.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2006.10.019