putzig is required for cell proliferation and regulates notch activity in Drosophila

We have identified the gene putzig (pzg) as a key regulator of cell proliferation and of Notch signaling in Drosophila. pzg encodes a Zn-finger protein that was found earlier within a macromolecular complex, including TATA-binding protein-related factor 2 (TRF2)/DNA replication-related element facto...

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Bibliographic Details
Published in:Molecular biology of the cell 2007-10, Vol.18 (10), p.3733-3740
Main Authors: Kugler, Sabrina J, Nagel, Anja C
Format: Article
Language:English
Subjects:
Animals
Cell Cycle
Cell Cycle Proteins - metabolism
Cell Proliferation
Chromatin - metabolism
Drosophila melanogaster - cytology
Drosophila melanogaster - growth & development
Drosophila melanogaster - metabolism
Drosophila Proteins - metabolism
Gene Expression Regulation, Developmental
Hedgehog Proteins - metabolism
Receptors, Notch - metabolism
Signal Transduction
Transcription Factors - metabolism
Transcriptional Activation
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Summary:We have identified the gene putzig (pzg) as a key regulator of cell proliferation and of Notch signaling in Drosophila. pzg encodes a Zn-finger protein that was found earlier within a macromolecular complex, including TATA-binding protein-related factor 2 (TRF2)/DNA replication-related element factor (DREF). This complex is involved in core promoter selection, where DREF functions as a transcriptional activator of replication-related genes. Here, we provide the first in vivo evidence that pzg is required for the expression of cell cycle and replication-related genes, and hence for normal developmental growth. Independent of its role in the TRF2/DREF complex, pzg acts as a positive regulator of Notch signaling that may occur by chromatin activation. Down-regulation of pzg activity inhibits Notch target gene activation, whereas Hedgehog (Hh) signal transduction and growth regulation are unaffected. Our findings uncover different modes of operation of pzg during imaginal development of Drosophila, and they provide a novel mechanism of Notch regulation.
ISSN:1059-1524
1939-4586
DOI:10.1091/mbc.E07-03-0263