Carcinoembryonic Antigen in Whole Serum

A microradioimmunoassay technique is described for detecting carcinoembryonic antigen (CEA) in whole serum. It differs from previous methods in being performed on 0·025 ml of whole serum instead of 5 ml of serum extracted with perchloric acid. The present assay was sufficiently sensitive to detect 8...

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Bibliographic Details
Published in:British journal of cancer 1972-10, Vol.26 (5), p.356-360
Main Authors: MacSween, J M, Warner, N L, Bankhurst, A D, Mackay, I R
Format: Article
Language:English
Subjects:
Ammonium Sulfate
Animals
Antigen-Antibody Complex
Antigens, Neoplasm - analysis
Biomedical and Life Sciences
Biomedicine
Breast Neoplasms - diagnosis
Cancer Research
Colonic Neoplasms - diagnosis
Drug Resistance
Epidemiology
False Positive Reactions
Female
gamma-Globulins
Gastrointestinal Neoplasms - diagnosis
Gastrointestinal Neoplasms - immunology
Goats
Humans
Liver Cirrhosis - diagnosis
Lung Neoplasms - diagnosis
Methods
Molecular Medicine
Oncology
original-article
Pancreatitis - diagnosis
Perchlorates
Rabbits - immunology
Radioimmunoassay
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Summary:A microradioimmunoassay technique is described for detecting carcinoembryonic antigen (CEA) in whole serum. It differs from previous methods in being performed on 0·025 ml of whole serum instead of 5 ml of serum extracted with perchloric acid. The present assay was sufficiently sensitive to detect 85% of carcinomata, localized to the colon, but positive results occurred also with certain non-gastrointestinal cancers, chiefly lung and breast, and certain non-malignant diseases. Many of the latter sera, with the general exception of alcoholic cirrhosis and pancreatitis, gave negative results after extraction with perchloric acid. It is suggested that a direct assay for CEA in whole serum may permit testing of large numbers of sera by laboratories with facilities for radioimmunoassays.
ISSN:0007-0920
1532-1827
DOI:10.1038/bjc.1972.46