Effects of insulin, human placental lactogen and human growth hormone on DNA synthesis in organ cultures of benign human breast tumours

Nineteen benign human breast tumours, from 19 premenopausal patients, were processed into slices and each tumour was individually cultured for 2 days in Medium 199. The effects of bovine insulin (5.0 microgram/ml) human placental lactogen (10.0 microgram/ml) and human growth hormone (10.0 microgram/...

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Bibliographic Details
Published in:British journal of cancer 1978-08, Vol.38 (2), p.258-262
Main Authors: Welsch, C W, Dombroske, S E, McManus, M J
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Breast Neoplasms - metabolism
Cancer Research
Cell Division
Cells, Cultured
DNA, Neoplasm - biosynthesis
Drug Resistance
Epidemiology
Female
Growth Hormone - pharmacology
Humans
Insulin - pharmacology
Menopause
Molecular Medicine
Oncology
original-article
Placental Lactogen - pharmacology
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Summary:Nineteen benign human breast tumours, from 19 premenopausal patients, were processed into slices and each tumour was individually cultured for 2 days in Medium 199. The effects of bovine insulin (5.0 microgram/ml) human placental lactogen (10.0 microgram/ml) and human growth hormone (10.0 microgram/ml) on 3H-thymidine incorporation into DNA were determined on the cultured tumour slices. Insulin and human placental lactogen significantly (P less than 0.01) increased the mean incorporation of [3H]TdR into DNA, whereas human growth hormone was ineffective. These results provide evidence that insulin and human placental lactogen, but not human growth hormone, may be important factors in the aetiology of benign human breast tumours.
ISSN:0007-0920
1532-1827
DOI:10.1038/bjc.1978.196