Construction of Agrobacterium strains by electroporation of genomic DNA and its utility in analysis of chromosomal virulence mutations

We have extended the technique of electroporation as a genetic tool for manipulating the Agrobacterium tumefaciens chromosome. We used this technique to introduce chromosomal DNA into recipient A. tumefaciens strains by electroporation and constructed isogenic chvE mutants that share the same chromo...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1994-11, Vol.60 (11), p.4192-4194
Main Authors: Charles, T.C. (McGill University, Ste-Anne-de-Bellevue, Quebec, Canada), Doty, S.L, Nester, E.W
Format: Article
Language:English
Subjects:
AGROBACTERIUM TUMEFACIENS
Agrobacterium tumefaciens - genetics
Agrobacterium tumefaciens - pathogenicity
Bacteria
Bacteriology
Biological and medical sciences
Deoxyribonucleic acid
DNA
DNA, Bacterial
ELECTROPORACION
ELECTROPORATION
Electroporation - methods
Fundamental and applied biological sciences. Psychology
GENE
GENES
GENETICA
Genetics
GENETIQUE
Genome, Bacterial
Microbiology
MUTACION
MUTATION
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
PLANTAS
PLANTE
PODER PATOGENO
POUVOIR PATHOGENE
TRANSFORMACION GENETICA
TRANSFORMATION GENETIQUE
Transformation, Bacterial - genetics
Virulence - genetics
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Summary:We have extended the technique of electroporation as a genetic tool for manipulating the Agrobacterium tumefaciens chromosome. We used this technique to introduce chromosomal DNA into recipient A. tumefaciens strains by electroporation and constructed isogenic chvE mutants that share the same chromosomal background but differ in their types of pTi (octopine or nopaline). Both nopaline and octopine pTi-carrying chvE mutants were deficient in vir regulon induction and exhibited similar reductions in host range
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.60.11.4192-4194.1994