Murine hematopoietic stem cells and progenitors express adrenergic receptors

• Published in: Journal of neuroimmunology 2007-05, Vol.186 (1), p.27-36
• Main Authors: Muthu, Kuzhali, Iyer, Sivaraman, He, L.-K, Szilagyi, Andrea, Gamelli, Richard L, Shankar, Ravi, Jones, Stephen B
• Format: Article
• Language: English
• Subjects: Adrenergic Agents - pharmacology; Allergy and Immunology; Alpha adrenergic receptors; Animals; Antigens, CD - metabolism; Beta adrenergic receptors; Bone marrow; Bone Marrow Cells - metabolism; Cell Differentiation - drug effects; Colony-Forming Units Assay - methods; Flow Cytometry - methods; Gene Expression - drug effects; Gene Expression - physiology; Hematopoietic stem cells; Hematopoietic Stem Cells - metabolism; Macrophage; Male; Mice; Microscopy, Confocal - methods; Myelopoiesis; Neurology; Progenitors; Receptors, Adrenergic - genetics; Receptors, Adrenergic - metabolism; Time Factors; TNF production; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 0165-5728; 1872-8421
• DOI: 10.1016/j.jneuroim.2007.02.007

Abstract Association between the nervous and immune system is well documented. Immune cells originate within the bone marrow that is innervated. Thermal injury induces adrenergic stimulation, augments monocytopoiesis and alters the β-adrenergic receptor (AR) profile of bone marrow monocyte committed progenitors. This provides an impetus to study AR expression in hematopoietic progenitors along myeloid lineage. Using FACS analysis and confocal microscopy, we report the expression of α1-, α2- and β2 -AR in enriched populations of ER-MP20+ and ER-MP12+ myeloid progenitors, CD117+ and CD34+ multi-potential progenitors and more importantly pluripotent stem cells suggesting a plausible role for catecholamine in hematopoietic development.