Effect of BCG on cell-mediated cytotoxicity and serum blocking factor during growth of rat hepatoma

Inbred rats were injected s.c. with cells of syngeneic hepatoma D23, D23 cells + BCG as a mixed inoculum, mixed inoculum one side and D23 alone contralaterally, or BCG alone. Their blood mononuclear cells were tested weekly for cytotoxicity against D23 target cells using a microcytotoxicity method a...

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Bibliographic Details
Published in:British journal of cancer 1976-06, Vol.33 (6), p.584-592
Main Author: Embleton, M J
Format: Article
Language:English
Subjects:
Animals
Antibodies, Neoplasm
Antigen-Antibody Complex
BCG Vaccine
Biomedical and Life Sciences
Biomedicine
Cancer Research
Carcinoma, Hepatocellular - immunology
correspondence
Cytotoxicity Tests, Immunologic
Drug Resistance
Epidemiology
Immunity, Cellular
Immunization
Leukocytes - immunology
Liver Neoplasms - immunology
Molecular Medicine
Mycobacterium bovis - immunology
Neoplasms, Experimental - immunology
Oncology
Rats
Splenectomy
Time Factors
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Summary:Inbred rats were injected s.c. with cells of syngeneic hepatoma D23, D23 cells + BCG as a mixed inoculum, mixed inoculum one side and D23 alone contralaterally, or BCG alone. Their blood mononuclear cells were tested weekly for cytotoxicity against D23 target cells using a microcytotoxicity method and their serum was tested for blocking activity against cytotoxicity by lymph node cells from immunized rats. Tumour growth was suppressed when BCG was in contact with tumour cells but tumours grew unhindered if the BCG was given contralaterally. All rats receiving tumour cells, either alone or mixed with BCG, developed cell-mediated cytotoxicity which remained until termination at 35 days. Rats receiving BCG alone showed slight initial cytotoxicity which disappeared after 7 days. Blocking factors appeared in the serum of rats which developed growing tumours but not in rats whose tumours were suppressed by contact with BCG. Splenectomized rats did not differ markedly from intact rats in the in vitro studies or in vivo . It is concluded that development of cell-mediated immunity and blocking factors depends more upon treatment with tumour cells and the subsequent behaviour of the tumour than upon treatment with BCG per se.
ISSN:0007-0920
1532-1827
DOI:10.1038/bjc.1976.95