Induction of dopamine D3 receptor expression as a mechanism of behavioral sensitization to levodopa

• Published in: Proceedings of the National Academy of Sciences - PNAS 1997-04, Vol.94 (7), p.3363-3367
• Main Authors: Bordet, R, Ridray, S, Carboni, S, Diaz, J, Sokoloff, P, Schwartz, J C
• Format: Article
• Language: English
• Subjects: Animal behavior; Animals; Autoradiography; Behavior, Animal - drug effects; Biological Sciences; Dynorphins - genetics; Gene Expression Regulation - drug effects; In Situ Hybridization; Levodopa - pharmacology; Male; Neurology; Neurons; Rats; Rats, Wistar; Receptors, Dopamine D2 - genetics; Receptors, Dopamine D3; Rodents; Substance P - genetics
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.94.7.3363

In rats with unilateral lesions of the nigrostriatal dopamine pathway with 6-hydroxydopamine, the motor stimulating effects of levodopa, an indirect dopamine receptor agonist, evidenced by contraversive rotations, become enhanced upon repeated intermittent administration. However, the mechanisms of this behavioral sensitization are essentially unknown. We show that development of sensitization is accompanied by a progressive appearance of D 3 receptor mRNA and binding sites, visualized by in situ hybridization and 7-[ 3 H]hydroxy- N , N -di- n -propyl-2-aminotetralin autoradiography, respectively, occurring in the denervated caudate putamen, a brain area from which this receptor subtype is normally absent. Development and decay of these two processes occur with closely parallel time courses, whereas there were no marked changes in D 1 or D 2 receptor mRNAs. D 3 receptor induction by levodopa is mediated by repeated D 1 receptor stimulation, since it is prevented by the antagonist SCH 33390 and mimicked by the agonist SKF 38393, but not by two D 2 receptor agonists. The enhanced behavioral response to levodopa is mediated by the newly synthesized D 3 receptor, since it is antagonized by nafadotride, a preferential D 3 receptor antagonist, in low dosage, which has no such effect before D 3 receptor induction. D 3 receptor induction and behavioral sensitization are also accompanied by a sustained enhancement of prodynorphin mRNA level and a progressively decreasing expression of the preprotachykinin gene. We propose that imbalance between dynorphin and substance P release from the same striatonigral motor efferent pathway, related to D 3 receptor induction, is responsible for behavioral sensitization. Parkinson disease 6-hydroxydopamine rotation behavior prodynorphin preprotachykinin