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Specific transcriptional responses induced by 8-methoxypsoralen and UVA in yeast
Abstract Treatment of eukaryotic cells with 8-methoxypsoralen plus UVA irradiation (8-MOP/UVA) induces pyrimidine monoadducts and interstrand crosslinks and initiates a cascade of events leading to cytotoxic, mutagenic and carcinogenic responses. Transcriptional activation plays an important part in...
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Published in: | FEMS yeast research 2007-09, Vol.7 (6), p.866-878 |
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Treatment of eukaryotic cells with 8-methoxypsoralen plus UVA irradiation (8-MOP/UVA) induces pyrimidine monoadducts and interstrand crosslinks and initiates a cascade of events leading to cytotoxic, mutagenic and carcinogenic responses. Transcriptional activation plays an important part in these responses. Our previous study in Saccharomyces cerevisiae showed that the repair of these lesions involves the transient formation of DNA double-strand breaks and the enhanced expression of landmark DNA damage response genes such as RAD51, RNR2 and DUN1, as well as the Mec1/Rad53 kinase signaling cascade. We have now used DNA microarrays to examine genome-wide transcriptional changes produced after induction of 8-MOP/UVA photolesions. We found that 128 genes were strongly induced and 29 genes strongly repressed. Modifications in gene expression concern numerous biological processes. Compared to other genotoxic treatments, c. 42% of the response genes were specific to 8-MOP/UVA treatment. In addition to common DNA damage response genes and genes induced by environmental stresses, a large fraction of 8-MOP/UVA response genes correspond to membrane-related functions. |
doi_str_mv | 10.1111/j.1567-1364.2007.00270.x |
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Treatment of eukaryotic cells with 8-methoxypsoralen plus UVA irradiation (8-MOP/UVA) induces pyrimidine monoadducts and interstrand crosslinks and initiates a cascade of events leading to cytotoxic, mutagenic and carcinogenic responses. Transcriptional activation plays an important part in these responses. Our previous study in Saccharomyces cerevisiae showed that the repair of these lesions involves the transient formation of DNA double-strand breaks and the enhanced expression of landmark DNA damage response genes such as RAD51, RNR2 and DUN1, as well as the Mec1/Rad53 kinase signaling cascade. We have now used DNA microarrays to examine genome-wide transcriptional changes produced after induction of 8-MOP/UVA photolesions. We found that 128 genes were strongly induced and 29 genes strongly repressed. Modifications in gene expression concern numerous biological processes. Compared to other genotoxic treatments, c. 42% of the response genes were specific to 8-MOP/UVA treatment. In addition to common DNA damage response genes and genes induced by environmental stresses, a large fraction of 8-MOP/UVA response genes correspond to membrane-related functions.</description><identifier>ISSN: 1567-1356</identifier><identifier>EISSN: 1567-1364</identifier><identifier>DOI: 10.1111/j.1567-1364.2007.00270.x</identifier><identifier>PMID: 17608707</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>8‐methoxypsoralen (8‐MOP) plus UVA ; Cell Cycle - drug effects ; Cell Cycle - radiation effects ; Cytotoxicity ; Deoxyribonucleic acid ; DNA ; DNA Damage ; DNA microarrays ; DNA Repair ; Gene expression ; Gene Expression Regulation, Fungal - drug effects ; Gene Expression Regulation, Fungal - radiation effects ; Genomes ; Genotoxicity ; Kinases ; Methoxsalen - pharmacology ; microarrays ; Oligonucleotide Array Sequence Analysis ; Photosensitizing Agents - pharmacology ; Rad51 Recombinase - metabolism ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - drug effects ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae - radiation effects ; Saccharomyces cerevisiae Proteins - metabolism ; Time Factors ; Transcription activation ; Ultraviolet radiation ; Ultraviolet Rays</subject><ispartof>FEMS yeast research, 2007-09, Vol.7 (6), p.866-878</ispartof><rights>2007 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved 2007</rights><rights>2007 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved</rights><rights>2007 Michèle Dardalhon Journal compilation © 2007 Federation of European Microbiological Societies 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5430-e3ce6d5c6c5752ab5de2955535e80dc17d46e0d0de6e088fa2b55ba522d4c6b93</citedby><cites>FETCH-LOGICAL-c5430-e3ce6d5c6c5752ab5de2955535e80dc17d46e0d0de6e088fa2b55ba522d4c6b93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17608707$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dardalhon, Michèle</creatorcontrib><creatorcontrib>Lin, Waka</creatorcontrib><creatorcontrib>Nicolas, Alain</creatorcontrib><creatorcontrib>Averbeck, Dietrich</creatorcontrib><title>Specific transcriptional responses induced by 8-methoxypsoralen and UVA in yeast</title><title>FEMS yeast research</title><addtitle>FEMS Yeast Res</addtitle><description>Abstract
Treatment of eukaryotic cells with 8-methoxypsoralen plus UVA irradiation (8-MOP/UVA) induces pyrimidine monoadducts and interstrand crosslinks and initiates a cascade of events leading to cytotoxic, mutagenic and carcinogenic responses. Transcriptional activation plays an important part in these responses. Our previous study in Saccharomyces cerevisiae showed that the repair of these lesions involves the transient formation of DNA double-strand breaks and the enhanced expression of landmark DNA damage response genes such as RAD51, RNR2 and DUN1, as well as the Mec1/Rad53 kinase signaling cascade. We have now used DNA microarrays to examine genome-wide transcriptional changes produced after induction of 8-MOP/UVA photolesions. We found that 128 genes were strongly induced and 29 genes strongly repressed. Modifications in gene expression concern numerous biological processes. Compared to other genotoxic treatments, c. 42% of the response genes were specific to 8-MOP/UVA treatment. In addition to common DNA damage response genes and genes induced by environmental stresses, a large fraction of 8-MOP/UVA response genes correspond to membrane-related functions.</description><subject>8‐methoxypsoralen (8‐MOP) plus UVA</subject><subject>Cell Cycle - drug effects</subject><subject>Cell Cycle - radiation effects</subject><subject>Cytotoxicity</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Damage</subject><subject>DNA microarrays</subject><subject>DNA Repair</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Fungal - drug effects</subject><subject>Gene Expression Regulation, Fungal - radiation effects</subject><subject>Genomes</subject><subject>Genotoxicity</subject><subject>Kinases</subject><subject>Methoxsalen - pharmacology</subject><subject>microarrays</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Photosensitizing Agents - pharmacology</subject><subject>Rad51 Recombinase - metabolism</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - drug effects</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae - radiation effects</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>Time Factors</subject><subject>Transcription activation</subject><subject>Ultraviolet radiation</subject><subject>Ultraviolet Rays</subject><issn>1567-1356</issn><issn>1567-1364</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>TOX</sourceid><sourceid>24P</sourceid><recordid>eNqNUV1r3DAQFKWl-Wj_QhD02c5KtiwflEII-YJAS9sU-iRkaS_R4bNcyU7O_75y7ri2lEL1sgs7M9qdIYQyyFl6p6uciUpmrKjKnAPIHIBLyDcvyOF-8HLfi-qAHMW4AmASoH5NDpisoJYgD8mnLz0at3SGDkF30QTXD853uqUBY--7iJG6zo4GLW0mWmdrHB78ZuqjD7rFjurO0rtvZwlEJ9RxeENeLXUb8e2uHpO7y4uv59fZ7cerm_Oz28yIsoAMC4OVFaYyQgquG2GRL4QQhcAarGHSlhWCBYup1PVS80aIRgvObWmqZlEckw9b3X5s1mgNdumAVvXBrXWYlNdO_Tnp3IO694-KQwmsngXe7QSC_zFiHNTKjyFdHhUvCsHZomQsoeotygQfY8Dl_gcGas5CrdRss5otV3MW6jkLtUnUk983_EXcmZ8A77eAJ9fi9N_C6vL759QkerGl-7H_Bzn7e6uf62eoUQ</recordid><startdate>200709</startdate><enddate>200709</enddate><creator>Dardalhon, Michèle</creator><creator>Lin, Waka</creator><creator>Nicolas, Alain</creator><creator>Averbeck, Dietrich</creator><general>Blackwell Publishing Ltd</general><general>Oxford University Press</general><scope>TOX</scope><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope></search><sort><creationdate>200709</creationdate><title>Specific transcriptional responses induced by 8-methoxypsoralen and UVA in yeast</title><author>Dardalhon, Michèle ; Lin, Waka ; Nicolas, Alain ; Averbeck, Dietrich</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5430-e3ce6d5c6c5752ab5de2955535e80dc17d46e0d0de6e088fa2b55ba522d4c6b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>8‐methoxypsoralen (8‐MOP) plus UVA</topic><topic>Cell Cycle - drug effects</topic><topic>Cell Cycle - radiation effects</topic><topic>Cytotoxicity</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Damage</topic><topic>DNA microarrays</topic><topic>DNA Repair</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Fungal - drug effects</topic><topic>Gene Expression Regulation, Fungal - radiation effects</topic><topic>Genomes</topic><topic>Genotoxicity</topic><topic>Kinases</topic><topic>Methoxsalen - pharmacology</topic><topic>microarrays</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Photosensitizing Agents - pharmacology</topic><topic>Rad51 Recombinase - metabolism</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - drug effects</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae - radiation effects</topic><topic>Saccharomyces cerevisiae Proteins - metabolism</topic><topic>Time Factors</topic><topic>Transcription activation</topic><topic>Ultraviolet radiation</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dardalhon, Michèle</creatorcontrib><creatorcontrib>Lin, Waka</creatorcontrib><creatorcontrib>Nicolas, Alain</creatorcontrib><creatorcontrib>Averbeck, Dietrich</creatorcontrib><collection>Open Access: Oxford University Press Open Journals</collection><collection>Open Access: Wiley-Blackwell Open Access Journals</collection><collection>Wiley Free Archive</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>FEMS yeast research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dardalhon, Michèle</au><au>Lin, Waka</au><au>Nicolas, Alain</au><au>Averbeck, Dietrich</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Specific transcriptional responses induced by 8-methoxypsoralen and UVA in yeast</atitle><jtitle>FEMS yeast research</jtitle><addtitle>FEMS Yeast Res</addtitle><date>2007-09</date><risdate>2007</risdate><volume>7</volume><issue>6</issue><spage>866</spage><epage>878</epage><pages>866-878</pages><issn>1567-1356</issn><eissn>1567-1364</eissn><abstract>Abstract
Treatment of eukaryotic cells with 8-methoxypsoralen plus UVA irradiation (8-MOP/UVA) induces pyrimidine monoadducts and interstrand crosslinks and initiates a cascade of events leading to cytotoxic, mutagenic and carcinogenic responses. Transcriptional activation plays an important part in these responses. Our previous study in Saccharomyces cerevisiae showed that the repair of these lesions involves the transient formation of DNA double-strand breaks and the enhanced expression of landmark DNA damage response genes such as RAD51, RNR2 and DUN1, as well as the Mec1/Rad53 kinase signaling cascade. We have now used DNA microarrays to examine genome-wide transcriptional changes produced after induction of 8-MOP/UVA photolesions. We found that 128 genes were strongly induced and 29 genes strongly repressed. Modifications in gene expression concern numerous biological processes. Compared to other genotoxic treatments, c. 42% of the response genes were specific to 8-MOP/UVA treatment. In addition to common DNA damage response genes and genes induced by environmental stresses, a large fraction of 8-MOP/UVA response genes correspond to membrane-related functions.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>17608707</pmid><doi>10.1111/j.1567-1364.2007.00270.x</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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subjects | 8‐methoxypsoralen (8‐MOP) plus UVA Cell Cycle - drug effects Cell Cycle - radiation effects Cytotoxicity Deoxyribonucleic acid DNA DNA Damage DNA microarrays DNA Repair Gene expression Gene Expression Regulation, Fungal - drug effects Gene Expression Regulation, Fungal - radiation effects Genomes Genotoxicity Kinases Methoxsalen - pharmacology microarrays Oligonucleotide Array Sequence Analysis Photosensitizing Agents - pharmacology Rad51 Recombinase - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae - radiation effects Saccharomyces cerevisiae Proteins - metabolism Time Factors Transcription activation Ultraviolet radiation Ultraviolet Rays |
title | Specific transcriptional responses induced by 8-methoxypsoralen and UVA in yeast |
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