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Specific transcriptional responses induced by 8-methoxypsoralen and UVA in yeast

Abstract Treatment of eukaryotic cells with 8-methoxypsoralen plus UVA irradiation (8-MOP/UVA) induces pyrimidine monoadducts and interstrand crosslinks and initiates a cascade of events leading to cytotoxic, mutagenic and carcinogenic responses. Transcriptional activation plays an important part in...

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Published in:FEMS yeast research 2007-09, Vol.7 (6), p.866-878
Main Authors: Dardalhon, Michèle, Lin, Waka, Nicolas, Alain, Averbeck, Dietrich
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Lin, Waka
Nicolas, Alain
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description Abstract Treatment of eukaryotic cells with 8-methoxypsoralen plus UVA irradiation (8-MOP/UVA) induces pyrimidine monoadducts and interstrand crosslinks and initiates a cascade of events leading to cytotoxic, mutagenic and carcinogenic responses. Transcriptional activation plays an important part in these responses. Our previous study in Saccharomyces cerevisiae showed that the repair of these lesions involves the transient formation of DNA double-strand breaks and the enhanced expression of landmark DNA damage response genes such as RAD51, RNR2 and DUN1, as well as the Mec1/Rad53 kinase signaling cascade. We have now used DNA microarrays to examine genome-wide transcriptional changes produced after induction of 8-MOP/UVA photolesions. We found that 128 genes were strongly induced and 29 genes strongly repressed. Modifications in gene expression concern numerous biological processes. Compared to other genotoxic treatments, c. 42% of the response genes were specific to 8-MOP/UVA treatment. In addition to common DNA damage response genes and genes induced by environmental stresses, a large fraction of 8-MOP/UVA response genes correspond to membrane-related functions.
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subjects 8‐methoxypsoralen (8‐MOP) plus UVA
Cell Cycle - drug effects
Cell Cycle - radiation effects
Cytotoxicity
Deoxyribonucleic acid
DNA
DNA Damage
DNA microarrays
DNA Repair
Gene expression
Gene Expression Regulation, Fungal - drug effects
Gene Expression Regulation, Fungal - radiation effects
Genomes
Genotoxicity
Kinases
Methoxsalen - pharmacology
microarrays
Oligonucleotide Array Sequence Analysis
Photosensitizing Agents - pharmacology
Rad51 Recombinase - metabolism
Saccharomyces cerevisiae
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae - radiation effects
Saccharomyces cerevisiae Proteins - metabolism
Time Factors
Transcription activation
Ultraviolet radiation
Ultraviolet Rays
title Specific transcriptional responses induced by 8-methoxypsoralen and UVA in yeast
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