Linking double-stranded DNA breaks to the recombination activating gene complex directs repair to the nonhomologous end-joining pathway

Two major DNA repair pathways, nonhomologous end-joining (NHEJ) and homologous recombination (HR), repair double-stranded DNA breaks (DSBs) in all eukaryotes. Additionally, several alternative end-joining pathways (or subpathways) have been reported that characteristically use short-sequence homolog...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2007-10, Vol.104 (43), p.17046-17051
Main Authors: Cui, Xiaoping, Meek, Katheryn
Format: Article
Language:English
Subjects:
Antigens, Nuclear
Biological Sciences
Cell Survival
Cellular biology
Cultured cells
Deoxyribonucleic acid
DNA
DNA Breaks, Double-Stranded
DNA Repair
DNA-Activated Protein Kinase - deficiency
DNA-Binding Proteins - deficiency
Double stranded DNA breaks
Eukaryotes
Genetic transposition
Homeodomain Proteins - metabolism
Ku Autoantigen
Mean absolute deviation
Multiprotein Complexes - metabolism
Oligonucleotides
Plasmids
Plasmids - metabolism
Protein Binding
Proteins
Recombination, Genetic
Yeasts
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Summary:Two major DNA repair pathways, nonhomologous end-joining (NHEJ) and homologous recombination (HR), repair double-stranded DNA breaks (DSBs) in all eukaryotes. Additionally, several alternative end-joining pathways (or subpathways) have been reported that characteristically use short-sequence homologies at the DNA ends to facilitate joining. How a cell chooses which DNA repair pathway to use (at any particular DSB) is a central and largely unanswered question. For one type of DSB, there is apparently no choice. DSBs mediated by the lymphocyte-specific recombination activating gene (RAG) endonuclease are repaired virtually exclusively by NHEJ. Here we demonstrate that non-RAG-mediated DSBs can be similarly forced into the NHEJ pathway by physical association with the RAG endonuclease.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0610928104