Multiple immunizations with adenovirus and MVA vectors improve CD8+ T cell functionality and mucosal homing

Abstract Recombinant adenovirus vectors and MVA vectors were used in prime boost vaccine regimens to address the impact of repeated immunizations on transgene product-specific CD8+ T cell frequencies, phenotypes, function, and localization. We show that a regimen with three immunizations incorporati...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2007-10, Vol.367 (1), p.156-167
Main Authors: Tatsis, Nia, Lin, Shih-Wen, Harris-McCoy, Kimberly, Garber, David A, Feinberg, Mark B, Ertl, Hildegund C.J
Format: Article
Language:English
Subjects:
Adenoviridae - genetics
Adenoviridae - metabolism
AIDS Vaccines - administration & dosage
AIDS Vaccines - genetics
Animals
CD8-Positive T-Lymphocytes - immunology
Female
Gene Products, gag - genetics
Gene Products, gag - immunology
Gene Products, gag - metabolism
Genetic Vectors
HIV-1
HIV-1 - genetics
HIV-1 - immunology
Humans
Immunization
Immunization Schedule
Immunization, Secondary
Infectious Disease
Intestinal Mucosa - cytology
Intestinal Mucosa - immunology
Lymph Nodes - cytology
Lymph Nodes - immunology
Lymphocyte Activation
Mice
Mice, Inbred BALB C
Peyer's Patches - cytology
Peyer's Patches - immunology
Prime Boost
Vaccine
Vaccinia virus - genetics
Vaccinia virus - metabolism
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Summary:Abstract Recombinant adenovirus vectors and MVA vectors were used in prime boost vaccine regimens to address the impact of repeated immunizations on transgene product-specific CD8+ T cell frequencies, phenotypes, function, and localization. We show that a regimen with three immunizations incorporating MVA, human adenovirus serotype 5 and chimpanzee-derived adenoviruses serotype 68 or 7 yields high transgene product-specific CD8+ T cell frequencies in spleen, blood, lymph nodes, and peritoneal lavage. Furthermore, upon triple immunization increased frequencies of transgene-specific T cells were measured at mucosal sites such as mesenteric lymph nodes, intestinal epithelium, and Peyer's patches. Multiple dose vaccine regimens that markedly increase functionally active transgene-specific T cells and target them to the appropriate ports of entry may be important in protection against pathogens such as HIV-1.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2007.05.028