A STUDY OF THE MUSCARINIC RECEPTOR BY GEL ELECTROPHORESIS

1 Muscarinic receptors from the brain of rat, guinea‐pig and frog have been labelled with tritiated propylbenzilylcholine mustard ([3H]‐PrBCM). 2 After solubilisation and sodium dodecyl sulphate (SDS)‐polyacrylamide gel electrophoresis a single radiolabeled peak was seen which was completely suppres...

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Bibliographic Details
Published in:British journal of pharmacology 1979-06, Vol.66 (2), p.337-342
Main Authors: BIRDSALL, N.J.M., BURGEN, A.S.V., HULME, E.C.
Format: Article
Language:English
Subjects:
Animals
Anura
Brain Chemistry
Electrophoresis, Polyacrylamide Gel - methods
Guinea Pigs
Ileum - analysis
Microsomes - analysis
Propylbenzilylcholine Mustard
Rats
Receptors, Cholinergic - analysis
Receptors, Muscarinic - analysis
Synaptic Membranes - analysis
Synaptosomes - analysis
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Summary:1 Muscarinic receptors from the brain of rat, guinea‐pig and frog have been labelled with tritiated propylbenzilylcholine mustard ([3H]‐PrBCM). 2 After solubilisation and sodium dodecyl sulphate (SDS)‐polyacrylamide gel electrophoresis a single radiolabeled peak was seen which was completely suppressed when labelling was carried out in the presence of 10−6 m atropine. The results suggest the presence of a single polypeptide chain with a molecular weight of ∼80,000. 3 In the case of intestinal smooth muscle multiple labelled peaks were found. Precautions against proteolysis led to the identification of a major peak with a molecular weight in the region of 80,000.
ISSN:0007-1188
1476-5381
DOI:10.1111/j.1476-5381.1979.tb13685.x