EFFECT OF 5‐HYDROXYTRYPTAMINE ON PROTEIN SYNTHESIS IN GASTROINTESTINAL AND OTHER TISSUES AND ON SERUM GASTRIN CONCENTRATIONS IN RATS

1 The effect of 5‐hydroxytryptamine (5‐HT) on protein synthesis in the gastrointestinal tissues as well as in the liver, heart and brain was studied by measuring [3H]‐leucine incorporation into total tissue protein in vivo in rats. 2 A single injection of 5‐HT (10 mg/kg) produced a marked inhibition...

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Bibliographic Details
Published in:British journal of pharmacology 1979-06, Vol.66 (2), p.211-215
Main Authors: MAJUMDAR, ADHIP P. NANDI, NAKHLA, ATIF M.
Format: Article
Language:English
Subjects:
Animals
Female
Gastric Mucosa - metabolism
Gastrins - blood
Intestinal Mucosa - metabolism
Intestines - drug effects
Male
Methysergide - pharmacology
Organ Specificity
Protein Biosynthesis - drug effects
Rats
Serotonin - pharmacology
Stomach - drug effects
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Summary:1 The effect of 5‐hydroxytryptamine (5‐HT) on protein synthesis in the gastrointestinal tissues as well as in the liver, heart and brain was studied by measuring [3H]‐leucine incorporation into total tissue protein in vivo in rats. 2 A single injection of 5‐HT (10 mg/kg) produced a marked inhibition (45 to 65%) in protein synthesis in the stomach (oxyntic gland area), intestine (jejunum + ileum), colon and brain, but not in the liver and heart. 3 Dose‐ and time‐dependent studies of 5‐HT‐induced changes in protein synthesis in the stomach of fed rats revealed that the maximal inhibition of about 65% occurred 1 h after a dose of 12.5 mg/kg. 4 Animals deprived of food for 24 h differed from their fed counterparts only in the degree of responsiveness, in that a greater inhibition (75%) of [3H]‐leucine incorporation into total protein of the stomach was observed 30 min after 5‐HT injection. 5 Pretreatment of animals with methysergide (0.25 mg/kg) essentially abolished the 5‐HT‐mediated inhibition of protein synthesis in the stomach. 6 Serum gastrin concentration in fasted animals remained slightly elevated during the initial period of 5‐HT treatment (up to 1 h). 7 The results demonstrate that in certain tissues, 5‐HT markedly inhibits protein synthesis. The inhibition in protein synthesis in the gastrointestinal tract cannot be attributed to changes in the concentration of gastrin.
ISSN:0007-1188
1476-5381
DOI:10.1111/j.1476-5381.1979.tb13667.x