ON THE RELEASE OF CATECHOLAMINES AND DOPAMINE‐β‐HYDROXYLASE EVOKED BY OUABAIN IN THE PERFUSED CAT ADRENAL GLAND

• Published in: British journal of pharmacology 1980-03, Vol.68 (3), p.571-583
• Main Authors: GARCIA, A.G., HERNANDEZ, M., HORGA, J.F., SANCHEZ‐GARCIA, P.
• Format: Article
• Language: English
• Subjects: Adrenal Glands - drug effects; Adrenal Glands - metabolism; Animals; Calcium - pharmacology; Catecholamines - metabolism; Cats; Dopamine beta-Hydroxylase - metabolism; In Vitro Techniques; Mecamylamine - pharmacology; Ouabain - pharmacology; Perfusion; Physostigmine - pharmacology; Sodium - pharmacology; Sodium-Potassium-Exchanging ATPase - antagonists & inhibitors
• ISSN: 0007-1188; 1476-5381
• DOI: 10.1111/j.1476-5381.1980.tb14573.x

1 Secretion of catecholamines (CA) and dopamine‐β‐hydroxylase (DBH) activity from the retrogradely perfused cat adrenal gland was studied following ouabain infusion. Perfusion with ouabain (10−4 m) for 10 min caused a gradual release of CA in the effluent which reached its peak 30 min after the ouabain pulse, and was maintained constant for at least 1 h. The effect of ouabain seemed to be irreversible. 2 Mecamylamine, while blocking the CA secretory effects of acetylcholine (ACh) perfusion, did not affect the secretion of CA evoked by ouabain. In denervated adrenal glands, ouabain‐induced CA secretion was similar to that in the contralateral, innervated gland. However, physostigmine perfusion potentiated the CA secretory effects of ouabain. 3 The release of CA evoked by ouabain was accompanied by a proportional release of DBH activity. The time course of appearance of DBH activity followed the pattern of CA release. 4 The CA and DBH outputs in response to a pulse of ouabain were suppressed in the absence of calcium. Calcium reintroduction to a calcium‐free perfused, ouabain‐treated gland not only restored but greatly potentiated the release of CA and DBH. The amplitude of the secretory response to calcium reintroduction in ouabain‐treated glands was proportional to the extracellular calcium concentration, and was antagonized by an external sodium‐deficient medium. 5 These data demonstrate that ouabain releases CA from the perfused cat adrenal gland by a calcium‐dependent exocytotic mechanism. The secretory effect of ouabain is not secondary to the release of ACh from cholinergic nerve terminals present in the adrenal gland, but due to a direct action on the chromaffin cell itself. In addition, the results suggest that this action is exerted through redistribution of monovalent cations secondary to the inhibition by the glycoside of the sodium pump. Such monovalent cation redistribution may cause a rise of intracellular ionized calcium levels through the activation of an internal sodium‐dependent calcium influx system probably located in the chromaffin cell membrane.