Locations of the envCD genes on the physical map of the Escherichia coli chromosome

We used a digoxigenin-labeled DNA fragment, isolated from plasmid pJK131 (5), to probe DNA from five lambda clones of the Kohara "miniset" library. Southern hybridization of the 926-bp HincII-EcoRI fragment, which covers the 3' end of envC and the 5' start of envD, was positive w...

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Bibliographic Details
Published in:Journal of bacteriology 1992-06, Vol.174 (11), p.3828-3829
Main Authors: KLEIN, J. R, PLAPP, R
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacteriology
Biological and medical sciences
Carrier Proteins
Chromosome Mapping
Escherichia coli
Escherichia coli - genetics
Escherichia coli Proteins
Fundamental and applied biological sciences. Psychology
Genome, Bacterial
Membrane Proteins - genetics
Metabolism. Enzymes
Microbiology
Multidrug Resistance-Associated Proteins
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Summary:We used a digoxigenin-labeled DNA fragment, isolated from plasmid pJK131 (5), to probe DNA from five lambda clones of the Kohara "miniset" library. Southern hybridization of the 926-bp HincII-EcoRI fragment, which covers the 3' end of envC and the 5' start of envD, was positive with clones 529 and 530, and a weak signal was obtained with clone 531, indicating that this clone contains only parts of the probe sequence. As expected, clones 528 and 532 did not hybridize. Thus, the envCD genes could be located on the Kohara physical map between kb 3481.5 and 3485.5, with the sequence reading left to right. The relative order of the genes on the E. coli W3110 physical map is mreB, fabE, panF, and envCD.
ISSN:0021-9193
1098-5530
DOI:10.1128/jb.174.11.3828-3829.1992