Alterations in DNA methylation are early, but not initial, events in ovarian tumorigenesis

We compared global levels of DNA methylation as well as methylation of a specific locus (MyoD1) in ovarian cystadenomas, ovarian tumours of low malignant potential (LMP) and ovarian carcinomas to investigate the association between changes in DNA methylation and ovarian tumour development. As we rea...

Full description

Saved in:
Bibliographic Details
Published in:British journal of cancer 1997-01, Vol.75 (3), p.396-402
Main Authors: Cheng, P, Schmutte, C, Cofer, KF, Felix, JC, Yu, MC, Dubeau, L
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Blotting, Southern
Cancer Research
Carcinoma - genetics
Carcinoma - pathology
Carcinoma - surgery
Chromatography, High Pressure Liquid
Cystadenoma - genetics
Cystadenoma - pathology
Cystadenoma - surgery
DNA Methylation
DNA Primers
DNA, Neoplasm - analysis
Drug Resistance
Epidemiology
experimental-oncology
Female
Female genital diseases
Gynecology. Andrology. Obstetrics
Humans
Medical sciences
Molecular Medicine
Oncology
Ovarian Neoplasms - genetics
Ovarian Neoplasms - pathology
Ovarian Neoplasms - surgery
Ovary - metabolism
Polymerase Chain Reaction
Polymorphism, Genetic
Receptors, Androgen - genetics
Trinucleotide Repeats
Tumors
X Chromosome
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We compared global levels of DNA methylation as well as methylation of a specific locus (MyoD1) in ovarian cystadenomas, ovarian tumours of low malignant potential (LMP) and ovarian carcinomas to investigate the association between changes in DNA methylation and ovarian tumour development. As we realized that cystadenomas showed different methylation patterns from both LMP tumours and carcinomas, we verified their monoclonal origin as a means of confirming their true neoplastic nature. High-pressure liquid chromatographic (HPLC) analyses showed that global methylation levels in LMP tumours and carcinomas were 21% and 25% lower than in cystadenomas respectively (P = 0.0001 by one-way variance analysis). Changes in the methylation status of the MyoD1 locus were not seen in any of ten cystadenomas analysed but were present in five of ten LMP tumours and in five of ten carcinomas (P = 0.03). These findings suggest that alterations in DNA methylation are absent (or at least not as extensive) in ovarian cystadenomas, but are present in LMP tumours, the phenotypic features of which are intermediate between those of benign and malignant ovarian tumours. The results also emphasize the merit of distinguishing ovarian LMP tumours from cystadenomas, in spite of their similar clinical characteristics.
ISSN:0007-0920
1532-1827
DOI:10.1038/bjc.1997.64