In macrophages, HIV-1 assembles into an intracellular plasma membrane domain containing the tetraspanins CD81, CD9, and CD53

In macrophages, HIV-1 has been shown to bud into intracellular structures that contain the late endosome marker CD63. We show that these organelles are not endosomes, but an internally sequestered plasma membrane domain. Using immunofluorescence microscopy and immunoelectron microscopy, we find that...

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Bibliographic Details
Published in:The Journal of cell biology 2007-04, Vol.177 (2), p.329-341
Main Authors: Deneka, Magdalena, Pelchen-Matthews, Annegret, Byland, Rahel, Ruiz-Mateos, Ezequiel, Marsh, Mark
Format: Article
Language:English
Subjects:
Antibodies
Antigens, CD - analysis
Antigens, Differentiation, T-Lymphocyte - analysis
Cell Line, Tumor
Cell membranes
Cells
Cellular biology
Endosomes
HIV
HIV-1 - physiology
Human immunodeficiency virus
Human immunodeficiency virus 1
Humans
Intracellular Membranes - chemistry
Intracellular Membranes - ultrastructure
Intracellular Membranes - virology
Leukocytes, Mononuclear
Macrophages
Macrophages - cytology
Macrophages - metabolism
Macrophages - virology
Membrane Glycoproteins - analysis
Membranes
Microscopy
P branes
Platelet Membrane Glycoproteins - analysis
Proteins
T lymphocytes
Tetraspanin 25
Tetraspanin 28
Tetraspanin 29
Tetraspanin 30
Viral morphology
Virions
Virus Assembly
Viruses
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Summary:In macrophages, HIV-1 has been shown to bud into intracellular structures that contain the late endosome marker CD63. We show that these organelles are not endosomes, but an internally sequestered plasma membrane domain. Using immunofluorescence microscopy and immunoelectron microscopy, we find that HIV-1 buds into a compartment that contains the tetraspanins CD81, CD9, and CD53. On uninfected macrophages, these proteins are seen at the cell surface and in intracellular vacuole-like structures with a complex content of vesicles and interconnected membranes that lack endosome markers, including CD63. Significantly, these structures are accessible to small tracers (horseradish peroxidase or ruthenium red) applied to cells at 4°C, indicating that they are connected to the cell surface. HIV assembles on, and accumulates within, these intracellular compartments. Furthermore, CD63 is recruited to the virus-containing structures and incorporated into virions. These results indicate that, in macrophages, HIV-1 exploits a previously undescribed intracellular plasma membrane domain to assemble infectious particles.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.200609050