Three-dimensional arrangement of F-actin in the contractile ring of fission yeast

The contractile ring, which is required for cytokinesis in animal and yeast cells, consists mainly of actin filaments. Here, we investigate the directionality of the filaments in fission yeast using myosin S1 decoration and electron microscopy. The contractile ring is composed of around 1,000 to 2,0...

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Bibliographic Details
Published in:The Journal of cell biology 2007-08, Vol.178 (5), p.765-771
Main Authors: Kamasaki, Tomoko, Osumi, Masako, Mabuchi, Issei
Format: Article
Language:English
Subjects:
Actins
Actins - chemistry
Actins - metabolism
Actins - ultrastructure
Anaphase
Animal cells
Cell membranes
Cells
Cytokines
Cytokinesis
Cytoskeleton - metabolism
Cytoskeleton - ultrastructure
Electron microscopes
Microfilaments
Mitosis
Schizosaccharomyces
Schizosaccharomyces - cytology
Schizosaccharomyces - metabolism
Schizosaccharomyces pombe
Schizosaccharomyces pombe Proteins - metabolism
Schizosaccharomyces pombe Proteins - ultrastructure
Yeast
Yeasts
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Summary:The contractile ring, which is required for cytokinesis in animal and yeast cells, consists mainly of actin filaments. Here, we investigate the directionality of the filaments in fission yeast using myosin S1 decoration and electron microscopy. The contractile ring is composed of around 1,000 to 2,000 filaments each around 0.6 μm in length. During the early stages of cytokinesis, the ring consists of two semicircular populations of parallel filaments of opposite directionality. At later stages, before contraction, the ring filaments show mixed directionality. We consider that the ring is initially assembled from a single site in the division plane and that filaments subsequently rearrange before contraction initiates.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.200612018