Rpb7p subunit of yeast RNA polymerase II plays roles in the two major cytoplasmic mRNA decay mechanisms

The steady-state level of mRNAs is determined by the balance between their synthesis by RNA polymerase II (Pol II) and their decay. In the cytoplasm, mRNAs are degraded by two major pathways; one requires decapping and 5' to 3' exonuclease activity and the other involves 3' to 5'...

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Bibliographic Details
Published in:The Journal of cell biology 2007-09, Vol.178 (7), p.1133-1143
Main Authors: Lotan, Rona, Goler-Baron, Vicky, Duek, Lea, Haimovich, Gal, Choder, Mordechai
Format: Article
Language:English
Subjects:
Cytoplasm - metabolism
Cytoplasmic Structures - metabolism
Genes
Genes, Fungal
Genetics
Green Fluorescent Proteins - metabolism
Multiprotein Complexes - metabolism
Mutation - genetics
Polyadenylation
Protein Binding
Protein Subunits - metabolism
Protein Transport
Recombinant Fusion Proteins - metabolism
Ribonucleic acid
RNA
RNA polymerase
RNA Polymerase II - metabolism
RNA Stability
RNA, Messenger - metabolism
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins - metabolism
Temperature
Two-Hybrid System Techniques
Yeast
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Summary:The steady-state level of mRNAs is determined by the balance between their synthesis by RNA polymerase II (Pol II) and their decay. In the cytoplasm, mRNAs are degraded by two major pathways; one requires decapping and 5' to 3' exonuclease activity and the other involves 3' to 5' degradation. Rpb7p is a Pol II subunit that shuttles between the nucleus and the cytoplasm. Here, we show that Rpb7p is involved in the two mRNA decay pathways and possibly couples them. Rpb7p stimulates the deadenylation stage required for execution of both pathways. Additionally, Rpb7p is both an active component of the P bodies, where decapping and 5' to 3' degradation occur, and is capable of affecting the P bodies function. Moreover, Rpb7p interacts with the decapping regulator Pat1p in a manner important for the mRNA decay machinery. Rpb7p is also involved in the second pathway, as it stimulates 3' to 5' degradation. Our genetic analyses suggest that Rpb7p plays two distinct roles in mRNA decay, which can both be uncoupled from Rpb7p's role in transcription. Thus, Rpb7p plays pivotal roles in determining mRNA levels.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.200701165