protein tyrosine phosphatase Pez regulates TGFβ, epithelial-mesenchymal transition, and organ development

Epithelial-mesenchymal transition (EMT), crucial during embryogenesis for new tissue and organ formation, is also considered to be a prerequisite to cancer metastasis. We report here that the protein tyrosine phosphatase Pez is expressed transiently in discrete locations in developing brain, heart,...

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Bibliographic Details
Published in:The Journal of cell biology 2007-09, Vol.178 (7), p.1223-1235
Main Authors: Wyatt, Leila, Wadham, Carol, Crocker, Lesley A, Lardelli, Michael, Khew-Goodall, Yeesim
Format: Article
Language:English
Subjects:
Blood cells
Cadherins
Cell lines
Cell nucleus
Embryos
Epithelial cells
Gene expression regulation
Heart valves
Messenger RNA
Organogenesis
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Summary:Epithelial-mesenchymal transition (EMT), crucial during embryogenesis for new tissue and organ formation, is also considered to be a prerequisite to cancer metastasis. We report here that the protein tyrosine phosphatase Pez is expressed transiently in discrete locations in developing brain, heart, pharyngeal arches, and somites in zebrafish embryos. We also find that Pez knock-down results in defects in these organs, indicating a crucial role in organogenesis. Overexpression of Pez in epithelial MDCK cells causes EMT, with a drastic change in cell morphology and function that is accompanied by changes in gene expression typical of EMT. Transfection of Pez induced TGFβ signaling, critical in developmental EMT with a likely role also in oncogenic EMT. In zebrafish, TGFβ3 is co- expressed with Pez in a number of tissues and its expression was lost from these tissues when Pez expression was knocked down. Together, our data suggest Pez plays a crucial role in organogenesis by inducing TGFβ and EMT.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.200705035