Genetic evidence for a functional relationship between Hsp104 and Hsp70

The phenotypes of single Hsp104 and Hsp70 mutants of the budding Yeast Saccharomyces cerevisiae provide no clue that these proteins are functionally related. Mutation of the HSP104 gene severely reduces the ability of cells to survive short exposures to extreme temperatures (thermotolerance) but has...

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Bibliographic Details
Published in:Journal of Bacteriology 1993-10, Vol.175 (20), p.6484-6491
Main Authors: Sanchez, Y, Parsell, D.A, Taulien, J, Vogel, J.L, Craig, E.A, Lindquist, S
Format: Article
Language:English
Subjects:
Action of physical and chemical agents
Bacteriology
Biological and medical sciences
CALOR
CHALEUR
DNA Mutational Analysis
ESTRES TERMICO
FENOTIPOS
Fundamental and applied biological sciences. Psychology
Fungal Proteins - chemistry
Fungal Proteins - genetics
GENE
Gene Expression Regulation, Fungal
GENES
Genes, Fungal
Genetics
Heat-Shock Proteins - genetics
Hot Temperature
INDICE DE CRECIMIENTO
Microbiology
MUTACION INDUCIDA
MUTANT
MUTANTES
MUTATION PROVOQUEE
Mycology
PHENOTYPE
PROTEINAS
PROTEINE
Proteins
RESISTANCE A LA TEMPERATURE
RESISTENCIA A LA TEMPERATURA
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - genetics
Sequence Deletion
STRESS THERMIQUE
TAUX DE CROISSANCE
Temperature
Yeast
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Summary:The phenotypes of single Hsp104 and Hsp70 mutants of the budding Yeast Saccharomyces cerevisiae provide no clue that these proteins are functionally related. Mutation of the HSP104 gene severely reduces the ability of cells to survive short exposures to extreme temperatures (thermotolerance) but has no effect on growth rates. On the other hand, mutations in the genes that encode Hsp70 proteins have significant effects on growth rates but do not reduce thermotolerance. The absence of a thermotolerance defect in S. cerevisiae Hsp70 mutants is puzzling, since the protein clearly plays an important role in thermotolerance in a variety of other organisms. In this report, examination of the phenotypes of combined Hsp104 and Hsp70 mutants uncovers similarities in the functions of Hsp104 and Hsp70 not previously apparent. In the absence of the Hsp104 protein, Hsp70 is very important for thermotolerance in S. cerevisiae, particularly at very early times after a temperature upshift. Similarly, Hsp104 plays a substantial role in vegetative growth under conditions of decreased Hsp70 protein levels. These results suggest a close functional relationship between Hsp104 and Hsp70
ISSN:0021-9193
1098-5530
1067-8832
DOI:10.1128/jb.175.20.6484-6491.1993