Production of histamine-like and prostaglandin-like substances from serum incubated with rat, dog, mouse or human tumours

When diluted serum was incubated at 37° with finely minced tumour tissue (from rat, dog, mouse or man) there was a fall of haemolytic complement titre (0-30 minutes), the production of a “histamine-like” material (30-90 minutes) and a prostaglandin-like “active lipid” (90-150 minutes). This latter w...

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Bibliographic Details
Published in:British journal of experimental pathology 1973-04, Vol.54 (2), p.203-221
Main Authors: Apps, M C, Cater, D B
Format: Article
Language:English
Subjects:
Acetates
Animals
Aspirin - pharmacology
Carcinoma, Hepatocellular - metabolism
Complement System Proteins
Dogs
Histamine - biosynthesis
Histamine - pharmacology
Humans
Hydrogen-Ion Concentration
Immune Sera
In Vitro Techniques
Indomethacin - pharmacology
Lipids - biosynthesis
Lipids - pharmacology
Liver - metabolism
Liver Neoplasms - metabolism
Mice
Muscle Contraction
Muscles - metabolism
Neoplasms, Experimental - metabolism
Prostaglandin Antagonists
Prostaglandins - biosynthesis
Prostaglandins - pharmacology
Rats
Stomach - drug effects
Time Factors
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Summary:When diluted serum was incubated at 37° with finely minced tumour tissue (from rat, dog, mouse or man) there was a fall of haemolytic complement titre (0-30 minutes), the production of a “histamine-like” material (30-90 minutes) and a prostaglandin-like “active lipid” (90-150 minutes). This latter was extracted with ethyl acetate at pH 3 and produced contraction of a rat stomach-fundus-strip preparation. Production of both types of activity was approximately proportional to the quantity of serum in the incubation mixture and to the fall in the haemolytic complement titre. With a constant amount of serum there was an optimum quantity of tumour, above which no further increase of active material was obtained. Aspirin or indomethacin added to the serum abolished the production of the “active lipid” but did not affect the “histamine-like” material. Inhibition of C′1 activity had a similar effect, but inhibition of C′3 abolished the production of both “histamine-like” and “prostaglandin-like” activity. When tumour was incubated with Tyrode's solution, both active fractions were present but their amount did not increase with time. When serum was incubated with liver or muscle from rat or guinea-pig, there was no “production” of either “histamine-like” or “prostaglandin-like” material.
ISSN:0007-1021