Effect of tumour cell-conditioned medium on endothelial macromolecular permeability and its correlation with collagen

Conditioned medium prepared from mouse melanoma B16 cells (B16-CM) increases the macromolecular permeability of bovine aortic, venous and human umbilical vein endothelial monolayer. Collagen, which is synthesised by endothelial cells, has an important function in regulating the permeability of endot...

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Bibliographic Details
Published in:British journal of cancer 1996-01, Vol.73 (1), p.24-28
Main Authors: Utoguchi, N, Mizuguchi, H, Dantakean, A, Makimoto, H, Wakai, Y, Tsutsumi, Y, Nakagawa, S, Mayumi, T
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Cancer Research
Capillary Permeability - drug effects
Cattle
Cell Membrane Permeability - drug effects
Cells, Cultured
Collagen - metabolism
Culture Media, Conditioned
Drug Resistance
Endothelium, Vascular - metabolism
Enzyme Inhibitors - pharmacology
Epidemiology
experimental-oncology
Extracellular Matrix - metabolism
Host-tumor relations. Immunology. Biological markers
Humans
Macromolecular Substances
Medical sciences
Melanoma, Experimental - metabolism
Metalloendopeptidases - antagonists & inhibitors
Mice
Molecular Medicine
Oncology
Phenanthrolines - pharmacology
Tumors
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Summary:Conditioned medium prepared from mouse melanoma B16 cells (B16-CM) increases the macromolecular permeability of bovine aortic, venous and human umbilical vein endothelial monolayer. Collagen, which is synthesised by endothelial cells, has an important function in regulating the permeability of endothelial monolayer. Briefly, low collagen content leads to hyperpermeable structure of the endothelial monolayer. In the present studies, we examined the relationship between the increase of endothelial permeability and content of synthesised collagen of endothelial cells cultured with B16-CM. The B16-CM reduced endothelial collagen content but did not digest collagen directly. Matrix metalloproteinase inhibitor, 1,10-phenanthroline, inhibited the increase in permeability due to addition of B16-CM. These data suggest that B16-CM acts on endothelial cells, stimulating the digestion of endothelial collagen, and that the reduced content of collagen leads to the hyperpermeability of the endothelial monolayer.
ISSN:0007-0920
1532-1827
DOI:10.1038/bjc.1996.5