DNA damage and repair in tumour and non-tumour tissues of mice induced by nicotinamide

In vivo DNA damage and repair was induced by nicotinamide (NAM) in adenotype 12 virus-induced mouse sarcoma A12B3 and sarcoma F inoculated into CBA mice. DNA damage, NAM and NAD concentrations were measured after in vivo exposure to NAM, in tumours and spleens by alkaline elution and by HPLC analysi...

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Bibliographic Details
Published in:British journal of cancer 1996-08, Vol.74 (3), p.368-373
Main Authors: Olsson, AR, Sheng, Y, Pero, RW, Chaplin, DJ, Horsman, MR
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Cancer Research
DNA - drug effects
DNA Damage
DNA Repair
Drug Resistance
Epidemiology
experimental-oncology
Medical sciences
Mice
Mice, Inbred CBA
Molecular Medicine
NAD - metabolism
Niacinamide - pharmacology
Oncology
Poly(ADP-ribose) Polymerase Inhibitors
Radiation therapy and radiosensitizing agent
Radiation-Sensitizing Agents - pharmacology
Sarcoma, Experimental - metabolism
Treatment with physical agents
Treatment. General aspects
Tumors
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Summary:In vivo DNA damage and repair was induced by nicotinamide (NAM) in adenotype 12 virus-induced mouse sarcoma A12B3 and sarcoma F inoculated into CBA mice. DNA damage, NAM and NAD concentrations were measured after in vivo exposure to NAM, in tumours and spleens by alkaline elution and by HPLC analysis. Our results indicate that NAM between 100-1000 mg kg-1 causes a high level of in vivo DNA strand breaks in tumours and normal tissues in mice bearing the immunogenic sarcoma A12B3 but not in the non-immunogenic sarcoma F. The repair process was also delayed by the NAM treatment probably owing to inhibition of the DNA repair enzyme, poly(ADP-ribose)polymerase, as evidenced by accumulation of NAM and NAD. These data are consistent with NAM having a mechanism of action as a radiosensitiser at least in part by DNA repair inhibition. In addition, it should also be considered that high doses of NAM might cause considerable complications to normal tissue in tumour-bearing individuals.
ISSN:0007-0920
1532-1827
DOI:10.1038/bjc.1996.367