In vitro studies on the potential use of 5-aminolaevulinic acid-mediated photodynamic therapy for gynaecological tumours

Results are reported on the sensitivity of various gynaecological tumour cell lines to 5-aminolaevulinic acid-induced protoporphyrin IX-sensitised photodynamic therapy (ALA-PDT) in vitro. All cell lines tested accumulated ALA-induced protoporphyrin IX (PpIX) and demonstrated good sensitivity to ALA-...

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Bibliographic Details
Published in:British journal of cancer 1996-09, Vol.74 (6), p.881-887
Main Authors: Rossi, FM, Campbell, DL, Pottier, RH, Kennedy, JC, Dickson, EF
Format: Article
Language:English
Subjects:
Aminolevulinic Acid - pharmacology
Aminolevulinic Acid - therapeutic use
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Cancer Research
Drug Resistance
Epidemiology
experimental-oncology
Female
Genital Neoplasms, Female - drug therapy
Genital Neoplasms, Female - metabolism
Genital system. Mammary gland
HeLa Cells
Humans
Medical sciences
Molecular Medicine
Oncology
Photochemotherapy
Protoporphyrins - metabolism
Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)
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Summary:Results are reported on the sensitivity of various gynaecological tumour cell lines to 5-aminolaevulinic acid-induced protoporphyrin IX-sensitised photodynamic therapy (ALA-PDT) in vitro. All cell lines tested accumulated ALA-induced protoporphyrin IX (PpIX) and demonstrated good sensitivity to ALA-PDT. Localisation of PpIX in the mitochondria was demonstrated by fluorescence microscopy. Subcellular damage following ALA-PDT was observed using transmission electron microscopy. This damage was localised initially to the mitochondria, with damage to membranes and the nucleus and complete loss of intracytoplasmic organisation being observed subsequently. There was no apparent difference in ALA-PDT response between a multidrug-resistant ovarian carcinoma cell line and its parent line. These results indicate that ALA-PDT has potential for application to therapy of gynaecological malignancies.
ISSN:0007-0920
1532-1827
DOI:10.1038/bjc.1996.452