Biomaterial-mediated retroviral gene transfer using self-assembled monolayers

Abstract Biomaterial-mediated gene delivery has recently emerged as a promising alternative to conventional gene transfer technologies that focus on direct delivery of viral vectors or DNA-polymer/matrix complexes. However, biomaterial-based strategies have primarily targeted transient gene expressi...

Full description

Saved in:
Bibliographic Details
Published in:Biomaterials 2007-12, Vol.28 (34), p.5121-5127
Main Authors: Gersbach, Charles A, Coyer, Sean R, Le Doux, Joseph M, García, Andrés J
Format: Article
Language:English
Subjects:
Adenovirus
Advanced Basic Science
Alkanethiol
Animals
Biocompatible Materials - chemistry
Dentistry
DNA - chemistry
Extracellular Matrix - metabolism
Fibroblast
Fibronectins - chemistry
Gene therapy
Gene transfer
Gene Transfer Techniques
Genetic engineering
Genetic Engineering - methods
Genetic Therapy - methods
Mice
Microscopy, Electron, Scanning
NIH 3T3 Cells
Plasmids - metabolism
Polylysine - chemistry
Polymers - chemistry
Retroviridae - genetics
Retroviridae - metabolism
Self-assembly
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Biomaterial-mediated gene delivery has recently emerged as a promising alternative to conventional gene transfer technologies that focus on direct delivery of viral vectors or DNA-polymer/matrix complexes. However, biomaterial-based strategies have primarily targeted transient gene expression vehicles, including plasmid DNA and adenovirus particles. This study expands on this work by characterizing biomaterial properties conducive to the surface immobilization of retroviral particles and subsequent transduction of mammalian cells at the cell-material interface. Self-assembled monolayers (SAMs) of functionally-terminated alkanethiols on gold were used to establish biomaterial surfaces of defined chemical composition. Gene transfer was observed to be greater than 90% on NH2 -terminated surfaces, approximately 50% on COOH-functionalized surfaces, and undetectable on CH3 -terminated SAMs, similar to controls of tissue culture-treated polystyrene. Gene delivery via the NH2 -SAM was further characterized as a function of retrovirus coating time, virus concentration, and cell seeding density. Finally, SAM-mediated gene delivery was comparable to fibronectin- and poly- l -lysine-based methods for gene transfer. This work is significant to establishing safe and effective gene therapy strategies, developing efficient methods for gene delivery, and supporting recent progress in the field of biomaterial-mediated gene transfer.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2007.07.047