Antigenic changes in lipopolysaccharide I of Rhizobium leguminosarum bv. viciae in root nodules of Vicia sativa subsp. nigra occur during release from infection threads

Three different monoclonal antibodies raised against the O antigen-containing lipopolysaccharide (LPS I) of free-living cells were used in an immunocytochemical study to follow the fate of LPS I on the outer membrane of Rhizobium leguminosarum bv. viciae 248 during the nodulation of Vicia sativa sub...

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Bibliographic Details
Published in:Journal of Bacteriology 1991-05, Vol.173 (10), p.3177-3183
Main Authors: Goosen-de Roo, L, de Maagd, R A, Lugtenberg, B J
Format: Article
Language:English
Subjects:
absorcion
absorption
Antibodies, Bacterial - immunology
Antibodies, Monoclonal - immunology
antigene
antigenos
antigens
Antigens, Bacterial - immunology
Bacteriology
Biological and medical sciences
cell structure
Endocytosis
Epitopes
estructura celular
flora microbiana
flore microbienne
formation de nodosites
Fundamental and applied biological sciences. Psychology
Immunohistochemistry
immunologie
immunology
inmunologia
lipopolisacaridos
lipopolysaccharide
lipopolysaccharides
Lipopolysaccharides - immunology
microbial flora
Microbiology
Microscopy, Electron
nodosite racinaire
nodulacion
nodulation
nodules
nudosidades radiculares
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Plants - microbiology
Rhizobium - immunology
rhizobium leguminosarum
root nodulation
root nodules
structure cellulaire
Symbiosis
Vicia sativa
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Summary:Three different monoclonal antibodies raised against the O antigen-containing lipopolysaccharide (LPS I) of free-living cells were used in an immunocytochemical study to follow the fate of LPS I on the outer membrane of Rhizobium leguminosarum bv. viciae 248 during the nodulation of Vicia sativa subsp. nigra. After immunogold labeling, the LPS I epitopes were detected on the outer membrane of bacteria present in infection threads throughout the nodule. Epitopes were not detectable on bacteria released from the infection thread. The data show that the LPS I epitopes present on rhizobia in infection droplets disappear shortly before or during endocytosis of the bacteria into the host plant cell cytoplasm. The abruptness of the change suggests an active degradation or modification of LPS I epitopes rather than only a repression of their synthesis.
ISSN:0021-9193
1098-5530
1067-8832
DOI:10.1128/jb.173.10.3177-3183.1991