The Fine Structure and Mode of Attachment of the Sheathed Flagellum of Vibrio metchnikovii

The sheathed flagellum of Vibrio metchnikovii was chosen for a study of the attachment of the flagellum to the bacterial cell. Normal and autolysed organisms and isolated flagella were studied by electron microscopy using the techniques of thin sectioning and negative staining. The sheath of the fla...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of cell biology 1963-08, Vol.18 (2), p.327-336
Main Authors: Glauert, Audrey M., Kerridge, D., Horne, R. W.
Format: Article
Language:English
Subjects:
Bacteria
Cell Membrane
Cell membranes
Cell Wall
Cell walls
Cells
Cytoplasm
Electron micrographs
Electrons
Flagella
Microscopy
Microscopy, Electron
Negative staining
Old Medline
Plasma sheaths
Vibrio
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The sheathed flagellum of Vibrio metchnikovii was chosen for a study of the attachment of the flagellum to the bacterial cell. Normal and autolysed organisms and isolated flagella were studied by electron microscopy using the techniques of thin sectioning and negative staining. The sheath of the flagellum has the same layered structure as the cell wall of the bacterium, and in favourable thin sections it appears that the sheath is a continuation of the cell wall. After autolysis the sheath is usually absent and the core of the flagellum has a diameter of 120 A. Electron micrographs of autolysed bacteria negatively stained with potassium phosphotungstate show that the core ends in a basal disc just inside the plasma membrane. The basal disc is about 350 A in diameter and is thus considerably smaller than the "basal granules" described previously by other workers.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.18.2.327