The Demonstration of the Succinic Dehydrogenase System in Bacillus subtilis Using Tetranitro-Blue Tetrazolium Combined with Techniques of Electron Microscopy

Activity of the succinic dehydrogenase system was studied in Bacillus subtilis utilizing combined techniques of cytochemistry and electron microscopy. Organisms were incubated in a medium containing tetranitro-blue tetrazolium (TNBT) which served as an electron acceptor. Enzymatic activity, as evide...

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Bibliographic Details
Published in:The Journal of cell biology 1965-10, Vol.27 (1), p.53-66
Main Authors: Sedar, Albert W., Burde, Ronald M.
Format: Article
Language:English
Subjects:
Bacillus subtilis
Bacillus subtilis - cytology
Bacillus subtilis - enzymology
Bacteria
Cell membranes
Cell walls
Coloring Agents
Cytoplasm
Dehydrogenases
Electron microscopy
Electrons
Flagella
Histocytochemistry
In Vitro Techniques
Microscopy, Electron
Organelles
Succinate Dehydrogenase - metabolism
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Summary:Activity of the succinic dehydrogenase system was studied in Bacillus subtilis utilizing combined techniques of cytochemistry and electron microscopy. Organisms were incubated in a medium containing tetranitro-blue tetrazolium (TNBT) which served as an electron acceptor. Enzymatic activity, as evidenced by deposition of TNBT-formazan, was found on membranous organelles associated with the cytoplasmic membrane and septal plasma membrane, the nuclear area, and the plasma membrane. Flagella, ∼190 A in diameter, with thorn-like projections protruded through the cell wall. Tangential-oblique sections of the cell wall showed many pores ∼ 220 A in diameter with a center-to-center spacing of ∼450 A.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.27.1.53