A Radioautographic Study with H3-Thymidine on Adrenal Medulla Nuclei of Rats Intermittently Exposed to Cold

A considerable decrease (24 to 40%) of DNA content per nucleus previously observed in the adrenal medulla of rats exposed intermittently to cold is followed by restoration to normal and supranormal values. This phenomenon has now been studied by use of H3-thymidine, which was given to normal rats, t...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of cell biology 1966-01, Vol.28 (1), p.9-19
Main Author: Viola-Magni, Maria Pia
Format: Article
Language:English
Subjects:
Adrenal medulla
Adrenal Medulla - cytology
Animals
Autoradiography
Cell Division
Cell nucleus
Cell Nucleus - metabolism
Cells
Cold Temperature
DNA - biosynthesis
Dyes
Histograms
In Vitro Techniques
Kidneys
Nucleic acid precursors
Photometry
Rats
Room temperature
Stromal cells
Thymidine - metabolism
Tritium
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A considerable decrease (24 to 40%) of DNA content per nucleus previously observed in the adrenal medulla of rats exposed intermittently to cold is followed by restoration to normal and supranormal values. This phenomenon has now been studied by use of H3-thymidine, which was given to normal rats, to rats exposed to cold, and to animals brought to room temperature after cold exposure. In the first two conditions, no significant labeling of nuclei was observed. In the third, labeling took place clearly in the 1st 3 days. The grain counts showed that the early labeled nuclei had more grains than those labeled later, indicating differences in the rate of DNA synthesis. A statistically significant correlation was found, on the same nuclei, between amount of Feulgen dye and number of grains. It is concluded that net synthesis of DNA takes place in the phase of recovery from cold. This fact is not related to cell division, as no mitoses could ever be detected, but rather to the cold-induced loss of DNA. Clear demonstration is thus given of a marked variation in the amount of DNA per nucleus in relation to the functional conditions of adrenal medulla cells.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.28.1.9