Affinity of Animal Cell Nucleoli for Normal Serum. Preliminary Characterization of Serum and Cell Components

Nucleoli of animal cells cultured in vitro are modified by a component of "nonimmune" animal serum. Modified nucleoli bind fluorescein-conjugated nonimmune serum proteins, as shown by calcium ion-dependent fluorescence. Analysis of serum indicates that the nucleolar-binding component is a...

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Bibliographic Details
Published in:The Journal of cell biology 1966-06, Vol.29 (3), p.461-473
Main Authors: Maisel, John C., Lytle, Ralph I.
Format: Article
Language:English
Subjects:
Alpha-Globulins - pharmacology
Animal cells
Animals
Blood Chemical Analysis
Blood Protein Electrophoresis
Blood Proteins - pharmacology
Cell culture techniques
Cell growth
Cell nucleolus
Cell Nucleolus - drug effects
Cells
Culture Techniques
Cultured cells
Curtains
Fluorescence
Glycoproteins - pharmacology
Haplorhini
Histocytochemistry
Humans
Kidney - growth & development
Kidney cells
Microscopy, Electron
Reactivity
Ultracentrifugation
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Summary:Nucleoli of animal cells cultured in vitro are modified by a component of "nonimmune" animal serum. Modified nucleoli bind fluorescein-conjugated nonimmune serum proteins, as shown by calcium ion-dependent fluorescence. Analysis of serum indicates that the nucleolar-binding component is a globulin, with an electrophoretic mobility in the same region as the slow alpha-1 component in pH 8.6 Veronal buffer. The component has a low sedimentation constant (2.4S), and appears to contain glycoprotein with relatively high sialic acid content (8.5%); the latter moiety may be essential to reaction with nucleoli. The nucleolar component reacting with this alpha globulin fraction appears to be a histonelike basic protein. Primary cultures of animal cells have been supported for 1 wk through attachment, spreading, and outgrowth from colonies to confluent monolayers in medium containing a nucleolar-reactive serum fraction as the only protein supplement.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.29.3.461