The Distribution of Inorganic Cations in Mouse Testis. Electron Microscope and Microprobe Analysis

For localization of pyroantimonate-precipitable cations, mouse testes were fixed with a saturated aqueous solution of potassium pyroantimonate (pH about 9.2, without addition of any conventional fixative), hardened with formaldehyde, and postosmicated. A good preservation of the cell membranes and o...

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Bibliographic Details
Published in:The Journal of cell biology 1971-02, Vol.48 (2), p.314-323
Main Authors: Kierszenbaum, Abraham L., Libanati, Cesar M., Tandler, Carlos J.
Format: Article
Language:English
Subjects:
Animals
Anions
Antimony
Calcium - analysis
Cations
Cell Membrane - chemistry
Cell membranes
Cell Nucleus - chemistry
Cells
Chemical Precipitation
Electron Probe Microanalysis
Formaldehyde
Histocytochemistry
Histological Techniques
Hydrogen-Ion Concentration
Magnesium - analysis
Male
Mice
Microscopy, Electron
Potassium
Precipitates
Seminiferous tubules
Sertoli cells
Sertoli Cells - chemistry
Sodium - analysis
Spermatids
Spermatozoa - chemistry
Staining and Labeling
Testes
Testis - chemistry
Testis - cytology
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Summary:For localization of pyroantimonate-precipitable cations, mouse testes were fixed with a saturated aqueous solution of potassium pyroantimonate (pH about 9.2, without addition of any conventional fixative), hardened with formaldehyde, and postosmicated. A good preservation of the cell membranes and over-all cell morphology is obtained as well as a consistent and reproducible localization of the insoluble antimonate salts of magnesium, calcium, and sodium. Four sites of prominent antimonate deposits are revealed, besides a more or less uniform distribution of the precipitates. These sites are: (a) In the walls of the seminiferous tubules, localized in two concentric layers corresponding to the inner and outer layers of the tubular wall; (b) Around the blood vessels and adjacent connective tissue; (c) At the area of contact between the Sertoli cell and spermatids, where a double line of precipitate surrounds the head of the mature spermatids; and (d) In the cell nuclei, disposed between regions of the condensed chromatin. The nucleus of mature spermatids did not show any sign of antimonate precipitation. The implications of this inorganic cation distribution are discussed with relation to their anionic counterparts, their localization in other animal and plant tissues, and the possibility that those sites may represent barriers to the free passage of ions.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.48.2.314