Studies on Primary Cultures of Differentiated Fetal Liver Cells

A method for culturing non- or slowly growing, differentiated fetal rat liver cells is described. It involves the use of collagenase as a digesting agent and of a selective medium deficient in arginine which suppresses the growth of nonparenchymal liver cells. Evidence is presented that surviving ce...

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Bibliographic Details
Published in:The Journal of cell biology 1972-03, Vol.52 (3), p.559-568
Main Authors: Leffert, H. L., Paul, D.
Format: Article
Language:English
Subjects:
3T3 cells
Animals
Arginine - biosynthesis
Blood
Cell culture techniques
Cell Differentiation
Cell growth
Cell lines
Cells
Cells, Cultured - metabolism
Complement Fixation Tests
Culture Media
Cultured cells
DNA - biosynthesis
Female
Fetus - metabolism
Fibroblasts - metabolism
Hepatectomy
Hepatocytes
In Vitro Techniques
Liver
Liver - cytology
Liver - embryology
Liver cells
Liver Regeneration
Methods
Microbial Collagenase
Microscopy, Phase-Contrast
Ornithine - metabolism
Pregnancy
Rats
Serum Albumin - biosynthesis
Serum Albumin - metabolism
Simian virus 40 - pathogenicity
Thymidine - metabolism
Tritium
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Summary:A method for culturing non- or slowly growing, differentiated fetal rat liver cells is described. It involves the use of collagenase as a digesting agent and of a selective medium deficient in arginine which suppresses the growth of nonparenchymal liver cells. Evidence is presented that surviving cells (a) retain liver-specific urea cycle functions measured by their capacity to transform ornithine into arginine, (b) synthesize DNA in glucose-deficient medium, and (c) synthesize and secrete albumin. This primary cell culture responds to partially hepatectomized rat serum and may be an appropriate assay system for the study of mechanisms which regulate liver regeneration.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.52.3.559