A Demonstration of Several Deoxyribonuclease Activities in Mammalian Cell Mitochondria

Extracts of purified mitochondria from adult rabbit liver and kidney have been prepared by lysis with Triton X-100. Such extracts contain deoxyribonuclease activity demonstrable at alkaline pH. Studies utilizing the effects of substrate variation, differing ionic strength, nucleoside di- and triphos...

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Bibliographic Details
Published in:The Journal of cell biology 1974-09, Vol.62 (3), p.695-706
Main Authors: Durphy, Michael, Manley, Paul N., Friedberg, Errol C.
Format: Article
Language:English
Subjects:
Acid Phosphatase - metabolism
Adenosine Triphosphate - pharmacology
Animals
Benzenesulfonates - pharmacology
Cell biology
Centrifugation, Density Gradient
Cytochromes
Deoxyribonucleases - metabolism
Diphosphates - pharmacology
DNA
DNA - metabolism
Electron Transport Complex IV - metabolism
Enzymes
Hydrogen-Ion Concentration
Kidney - cytology
Kidneys
Liver
Lysosomes - enzymology
Mercury - pharmacology
Mitochondria
Mitochondria - enzymology
Mitochondria, Liver - enzymology
Nucleotides
Organometallic Compounds - pharmacology
Phosphatases
Potassium Chloride - pharmacology
Proteins - metabolism
Rabbits
Tritium
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Summary:Extracts of purified mitochondria from adult rabbit liver and kidney have been prepared by lysis with Triton X-100. Such extracts contain deoxyribonuclease activity demonstrable at alkaline pH. Studies utilizing the effects of substrate variation, differing ionic strength, nucleoside di- and triphosphates, and SH-group inhibitors reveal the existence of at least five distinguishable deoxyribonuclease activities in these extracts. Assay of lysosomal and mitochondrial enzyme markers indicates no significant lysosomal contamination of the mitochondrial extracts. Further studies also suggest that the alkaline deoxyribonuclease activity is specifically located in or in association with mitochondria.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.62.3.695