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A Demonstration of Several Deoxyribonuclease Activities in Mammalian Cell Mitochondria
Extracts of purified mitochondria from adult rabbit liver and kidney have been prepared by lysis with Triton X-100. Such extracts contain deoxyribonuclease activity demonstrable at alkaline pH. Studies utilizing the effects of substrate variation, differing ionic strength, nucleoside di- and triphos...
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| Published in: | The Journal of cell biology 1974-09, Vol.62 (3), p.695-706 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c400t-d7310b35060c299f0ef8f4e60a812a23f69a6f67f672ee3f9ba73d1b7db9b32a3 |
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| container_end_page | 706 |
| container_issue | 3 |
| container_start_page | 695 |
| container_title | The Journal of cell biology |
| container_volume | 62 |
| creator | Durphy, Michael Manley, Paul N. Friedberg, Errol C. |
| description | Extracts of purified mitochondria from adult rabbit liver and kidney have been prepared by lysis with Triton X-100. Such extracts contain deoxyribonuclease activity demonstrable at alkaline pH. Studies utilizing the effects of substrate variation, differing ionic strength, nucleoside di- and triphosphates, and SH-group inhibitors reveal the existence of at least five distinguishable deoxyribonuclease activities in these extracts. Assay of lysosomal and mitochondrial enzyme markers indicates no significant lysosomal contamination of the mitochondrial extracts. Further studies also suggest that the alkaline deoxyribonuclease activity is specifically located in or in association with mitochondria. |
| doi_str_mv | 10.1083/jcb.62.3.695 |
| format | article |
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Such extracts contain deoxyribonuclease activity demonstrable at alkaline pH. Studies utilizing the effects of substrate variation, differing ionic strength, nucleoside di- and triphosphates, and SH-group inhibitors reveal the existence of at least five distinguishable deoxyribonuclease activities in these extracts. Assay of lysosomal and mitochondrial enzyme markers indicates no significant lysosomal contamination of the mitochondrial extracts. Further studies also suggest that the alkaline deoxyribonuclease activity is specifically located in or in association with mitochondria.</description><identifier>ISSN: 0021-9525</identifier><identifier>EISSN: 1540-8140</identifier><identifier>DOI: 10.1083/jcb.62.3.695</identifier><identifier>PMID: 4368324</identifier><language>eng</language><publisher>United States: Rockefeller University Press</publisher><subject>Acid Phosphatase - metabolism ; Adenosine Triphosphate - pharmacology ; Animals ; Benzenesulfonates - pharmacology ; Cell biology ; Centrifugation, Density Gradient ; Cytochromes ; Deoxyribonucleases - metabolism ; Diphosphates - pharmacology ; DNA ; DNA - metabolism ; Electron Transport Complex IV - metabolism ; Enzymes ; Hydrogen-Ion Concentration ; Kidney - cytology ; Kidneys ; Liver ; Lysosomes - enzymology ; Mercury - pharmacology ; Mitochondria ; Mitochondria - enzymology ; Mitochondria, Liver - enzymology ; Nucleotides ; Organometallic Compounds - pharmacology ; Phosphatases ; Potassium Chloride - pharmacology ; Proteins - metabolism ; Rabbits ; Tritium</subject><ispartof>The Journal of cell biology, 1974-09, Vol.62 (3), p.695-706</ispartof><rights>Copyright 1974 The Rockefeller University Press</rights><rights>Copyright © 1974 by The Rockefeller University Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-d7310b35060c299f0ef8f4e60a812a23f69a6f67f672ee3f9ba73d1b7db9b32a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/1607215$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/1607215$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,58213,58446</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4368324$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Durphy, Michael</creatorcontrib><creatorcontrib>Manley, Paul N.</creatorcontrib><creatorcontrib>Friedberg, Errol C.</creatorcontrib><title>A Demonstration of Several Deoxyribonuclease Activities in Mammalian Cell Mitochondria</title><title>The Journal of cell biology</title><addtitle>J Cell Biol</addtitle><description>Extracts of purified mitochondria from adult rabbit liver and kidney have been prepared by lysis with Triton X-100. Such extracts contain deoxyribonuclease activity demonstrable at alkaline pH. Studies utilizing the effects of substrate variation, differing ionic strength, nucleoside di- and triphosphates, and SH-group inhibitors reveal the existence of at least five distinguishable deoxyribonuclease activities in these extracts. Assay of lysosomal and mitochondrial enzyme markers indicates no significant lysosomal contamination of the mitochondrial extracts. Further studies also suggest that the alkaline deoxyribonuclease activity is specifically located in or in association with mitochondria.</description><subject>Acid Phosphatase - metabolism</subject><subject>Adenosine Triphosphate - pharmacology</subject><subject>Animals</subject><subject>Benzenesulfonates - pharmacology</subject><subject>Cell biology</subject><subject>Centrifugation, Density Gradient</subject><subject>Cytochromes</subject><subject>Deoxyribonucleases - metabolism</subject><subject>Diphosphates - pharmacology</subject><subject>DNA</subject><subject>DNA - metabolism</subject><subject>Electron Transport Complex IV - metabolism</subject><subject>Enzymes</subject><subject>Hydrogen-Ion Concentration</subject><subject>Kidney - cytology</subject><subject>Kidneys</subject><subject>Liver</subject><subject>Lysosomes - enzymology</subject><subject>Mercury - pharmacology</subject><subject>Mitochondria</subject><subject>Mitochondria - enzymology</subject><subject>Mitochondria, Liver - enzymology</subject><subject>Nucleotides</subject><subject>Organometallic Compounds - pharmacology</subject><subject>Phosphatases</subject><subject>Potassium Chloride - pharmacology</subject><subject>Proteins - metabolism</subject><subject>Rabbits</subject><subject>Tritium</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1974</creationdate><recordtype>article</recordtype><recordid>eNpVkUtP3DAUhS3UCgbKjmUrZcWKTK8fcZJNpdHwqgTqgsLWcpzr4lFiUzszKv8eoxkBla50F-fTuY9DyAmFOYWGf1-Zbi7ZnM9lW-2RGa0ElA0V8InMABgt24pVB-QwpRUAiFrwfbIvuGw4EzPysCjOcQw-TVFPLvgi2OIONxj1kIXw7zm6Lvi1GVAnLBZmchs3OUyF88WtHkc9OO2LJQ5DceumYB6D76PTX8hnq4eEx7t-RO4vL34vr8ubX1c_l4ub0giAqexrTqHjFUgwrG0toG2sQAm6oUwzbmWrpZV1LobIbdvpmve0q_uu7TjT_Ij82Po-rbsRe4M-3zGop-hGHZ9V0E79r3j3qP6EjWIUWsZENjjdGcTwd41pUqNLJp-jPYZ1Ug0TAiRrMni2BU0MKUW0b0MoqNccVM5BSaa4yjlk_NvHxd7g3eOz_nWrr9IU4ruXhJrRir8A9xmPIQ</recordid><startdate>19740901</startdate><enddate>19740901</enddate><creator>Durphy, Michael</creator><creator>Manley, Paul N.</creator><creator>Friedberg, Errol C.</creator><general>Rockefeller University Press</general><general>The Rockefeller University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19740901</creationdate><title>A Demonstration of Several Deoxyribonuclease Activities in Mammalian Cell Mitochondria</title><author>Durphy, Michael ; Manley, Paul N. ; Friedberg, Errol C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-d7310b35060c299f0ef8f4e60a812a23f69a6f67f672ee3f9ba73d1b7db9b32a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1974</creationdate><topic>Acid Phosphatase - metabolism</topic><topic>Adenosine Triphosphate - pharmacology</topic><topic>Animals</topic><topic>Benzenesulfonates - pharmacology</topic><topic>Cell biology</topic><topic>Centrifugation, Density Gradient</topic><topic>Cytochromes</topic><topic>Deoxyribonucleases - metabolism</topic><topic>Diphosphates - pharmacology</topic><topic>DNA</topic><topic>DNA - metabolism</topic><topic>Electron Transport Complex IV - metabolism</topic><topic>Enzymes</topic><topic>Hydrogen-Ion Concentration</topic><topic>Kidney - cytology</topic><topic>Kidneys</topic><topic>Liver</topic><topic>Lysosomes - enzymology</topic><topic>Mercury - pharmacology</topic><topic>Mitochondria</topic><topic>Mitochondria - enzymology</topic><topic>Mitochondria, Liver - enzymology</topic><topic>Nucleotides</topic><topic>Organometallic Compounds - pharmacology</topic><topic>Phosphatases</topic><topic>Potassium Chloride - pharmacology</topic><topic>Proteins - metabolism</topic><topic>Rabbits</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Durphy, Michael</creatorcontrib><creatorcontrib>Manley, Paul N.</creatorcontrib><creatorcontrib>Friedberg, Errol C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Durphy, Michael</au><au>Manley, Paul N.</au><au>Friedberg, Errol C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Demonstration of Several Deoxyribonuclease Activities in Mammalian Cell Mitochondria</atitle><jtitle>The Journal of cell biology</jtitle><addtitle>J Cell Biol</addtitle><date>1974-09-01</date><risdate>1974</risdate><volume>62</volume><issue>3</issue><spage>695</spage><epage>706</epage><pages>695-706</pages><issn>0021-9525</issn><eissn>1540-8140</eissn><abstract>Extracts of purified mitochondria from adult rabbit liver and kidney have been prepared by lysis with Triton X-100. Such extracts contain deoxyribonuclease activity demonstrable at alkaline pH. Studies utilizing the effects of substrate variation, differing ionic strength, nucleoside di- and triphosphates, and SH-group inhibitors reveal the existence of at least five distinguishable deoxyribonuclease activities in these extracts. Assay of lysosomal and mitochondrial enzyme markers indicates no significant lysosomal contamination of the mitochondrial extracts. Further studies also suggest that the alkaline deoxyribonuclease activity is specifically located in or in association with mitochondria.</abstract><cop>United States</cop><pub>Rockefeller University Press</pub><pmid>4368324</pmid><doi>10.1083/jcb.62.3.695</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9525 |
| ispartof | The Journal of cell biology, 1974-09, Vol.62 (3), p.695-706 |
| issn | 0021-9525 1540-8140 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2109224 |
| source | JSTOR Journals and Primary Sources |
| subjects | Acid Phosphatase - metabolism Adenosine Triphosphate - pharmacology Animals Benzenesulfonates - pharmacology Cell biology Centrifugation, Density Gradient Cytochromes Deoxyribonucleases - metabolism Diphosphates - pharmacology DNA DNA - metabolism Electron Transport Complex IV - metabolism Enzymes Hydrogen-Ion Concentration Kidney - cytology Kidneys Liver Lysosomes - enzymology Mercury - pharmacology Mitochondria Mitochondria - enzymology Mitochondria, Liver - enzymology Nucleotides Organometallic Compounds - pharmacology Phosphatases Potassium Chloride - pharmacology Proteins - metabolism Rabbits Tritium |
| title | A Demonstration of Several Deoxyribonuclease Activities in Mammalian Cell Mitochondria |
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